Overview

  • Product name

  • Description

    Goat polyclonal to SIGLEC8
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-SYKSQLNYKTKQ

    , corresponding to internal sequence amino acids 134-145 of Human SIGLEC8 (NP_055257.2)

  • Positive control

    • MOLT4 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab79285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
    • Tissue specificity

      Expressed specifically on eosinophils.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD329 antigen antibody
      • CDw329 antibody
      • SAF 2 antibody
      • SAF-2 antibody
      • SAF2 antibody
      • Sialic acid binding Ig like lectin 8 antibody
      • Sialic acid-binding Ig-like lectin 8 antibody
      • Sialoadhesin family member 2 antibody
      • SIGL8_HUMAN antibody
      • Siglec 8 antibody
      • Siglec-8 antibody
      • SIGLEC8 antibody
      • SIGLEC8L antibody
      see all

    Images

    • Anti-SIGLEC8 antibody (ab79285) at 0.3 µg/ml + MOLT4 lysate (in RIPA buffer) at 35 µg

      Predicted band size: 54 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?



      The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 Jun 15;101(12):5014-20.; PMID: 12609831).

    References

    ab79285 has not yet been referenced specifically in any publications.

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