Anti-SIGLEC8 antibody - C-terminal (ab198690)
Key features and details
- Rabbit polyclonal to SIGLEC8 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SIGLEC8 antibody - C-terminal
See all SIGLEC8 primary antibodies -
Description
Rabbit polyclonal to SIGLEC8 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SIGLEC8 (C terminal).
Database link: Q9NYZ4 -
Positive control
- WB: Human liver lysate; IHC: Human liver and prostate cancer tissue.
-
General notes
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (2) |
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 54 kDa.
|
Notes |
---|
IHC-P
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/2000. Predicted molecular weight: 54 kDa. |
Target
-
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. -
Tissue specificity
Expressed specifically on eosinophils. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 27181 Human
- Omim: 605639 Human
- SwissProt: Q9NYZ4 Human
- Unigene: 447899 Human
-
Alternative names
- CD329 antigen antibody
- CDw329 antibody
- SAF 2 antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using ab198690 at dilution 1/75.
-
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using ab198690 at dilution 1/75.
-
Anti-SIGLEC8 antibody - C-terminal (ab198690) at 1/500 dilution + Human liver tissue lysate at 40 µg
Secondary
Goat anti rabbit IgG at 1/5000 dilution
Predicted band size: 54 kDa
Exposure time: 2 minutesGel: 8%SDS-PAGE
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab198690 has been referenced in 1 publication.
- Morshed N et al. Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration. Mol Syst Biol 16:e9819 (2020). PubMed: 33289969