The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/50 - 1/100. Predicted molecular weight: 65 kDa.
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Isoform Short is highly expressed in spleen, small intestine and adrenal gland; it is lower expressed in thyroid, placenta, brain, stomach, bone marrow, spinal chord and breast. Isoform Long is highly expressed in spleen, small intestine and bone marrow; it is lower expressed in thyroid, placenta, thymus, trachea, stomach, lung, adrenal gland, fetal brain and testis.
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human spleen tissue, labeling SIGLECL1 using ab172940 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-SIGLECL1 antibody - C-terminal (ab172940)
Anti-SIGLECL1 antibody - C-terminal (ab172940) at 1/50 dilution + mouse bladder tissue lysate at 35 µg