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  1. Link

    sigma-32-antibody-ab26890.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Sigma 32 antibody (ab26890)

  • Datasheet
Reviews (1) Submit a question References (1)

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Western blot - Anti-Sigma 32 antibody (ab26890)

    Key features and details

    • Rabbit polyclonal to Sigma 32
    • Suitable for: WB
    • Reacts with: Species independent
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Sigma 32 antibody
    • Description

      Rabbit polyclonal to Sigma 32
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Full length Sigma 32 protein (Serratia marcescens)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Lyophilized:Add 100ul sterile water.
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Heat Shock Proteins
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Microbiology
      • Organism
      • Bacteria
      • Gram negative Bacteria
      • Other gram-ve bacteria

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab26890 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
    Notes
    WB
    1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

    Target

    • Relevance

      When cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes.
    • Alternative names

      • RNA polymerase sigma 32 factor antibody
      • rpo H antibody
      • rpoH antibody

    Images

    • Western blot - Anti-Sigma 32 antibody (ab26890)
      Western blot - Anti-Sigma 32 antibody (ab26890)
      Lane 1 : Pre-bleed at 1/2000 dilution
      Lane 2 : Anti-serum at 1/2000 dilution

      All lanes : Antigen

      Lysates/proteins at 4 µg per lane.

      Secondary
      All lanes : anti-rabbit IgG-HRP

      Developed using the ECL technique.

      Predicted band size: 32 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab26890? Please let us know so that we can cite the reference in this datasheet.

    ab26890 has been referenced in 1 publication.

    • Mahalakshmi S  et al. yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli. Mol Microbiol 91:145-57 (2014). PubMed: 24266962

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Sigma 32 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10.9912 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Escherichia coli Cell lysate - whole cell (no specification)
    Specification
    no specification
    Treatment
    100 ´M Nalidixic Acid for 18 hours
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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