Anti-Sigma 32 antibody (ab26890)
Key features and details
- Rabbit polyclonal to Sigma 32
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Sigma 32 antibody -
Description
Rabbit polyclonal to Sigma 32 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length Sigma 32 protein (Serratia marcescens)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Add 100ul sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
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| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa). |
Target
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Relevance
When cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes. -
Alternative names
- RNA polymerase sigma 32 factor antibody
- rpo H antibody
- rpoH antibody
Images
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Western blot - Anti-Sigma 32 antibody (ab26890)Lane 1 : Pre-bleed at 1/2000 dilution
Lane 2 : Anti-serum at 1/2000 dilution
All lanes : Antigen
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : anti-rabbit IgG-HRP
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab26890 has been referenced in 1 publication.
- Mahalakshmi S et al. yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli. Mol Microbiol 91:145-57 (2014). PubMed: 24266962
