Product nameAnti-Sigma 32 antibody
DescriptionRabbit polyclonal to Sigma 32
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Full length Sigma 32 protein (Serratia marcescens)
FormLyophilised:Add 100ul sterile water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab26890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).|
RelevanceWhen cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes.
- RNA polymerase sigma 32 factor antibody
- rpo H antibody
- rpoH antibody
Lane 1 : Pre-bleed at 1/2000 dilution
Lane 2 : Anti-serum at 1/2000 dilution
All lanes : Antigen
Lysates/proteins at 4 µg per lane.
All lanes : anti-rabbit IgG-HRP
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab26890 has been referenced in 1 publication.
- Mahalakshmi S et al. yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli. Mol Microbiol 91:145-57 (2014). PubMed: 24266962