- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-Sigma 32 antibody
-
DescriptionRabbit polyclonal to Sigma 32
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with Serratia marcescens. Not yet tested in other species.
-
Immunogen
Full length Sigma 32 protein (Serratia marcescens)
Properties
-
FormLyophilised:Add 100ul sterile water.
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPBS buffer.
-
Concentration information loading... -
PurityProtein A purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab26890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa). |
Target
-
RelevanceWhen cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes.
-
Alternative names
- RNA polymerase sigma 32 factor antibody
- rpo H antibody
- rpoH antibody
Images
-
Western blot - Anti-Sigma 32 antibody (ab26890)Lane 1 : Pre-bleed at 1/2000 dilution
Lane 2 : Anti-serum at 1/2000 dilution
All lanes : Antigen
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : anti-rabbit IgG-HRP
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 38 kDa (why is the actual band size different from the predicted?)
Protocols
References
ab26890 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"