Anti-Sigma 32 antibody (ab26890)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Sigma 32 antibody -
Description
Rabbit polyclonal to Sigma 32 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length Sigma 32 protein (Serratia marcescens)
Properties
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Form
Lyophilised:Add 100ul sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab26890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa). |
Target
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Relevance
When cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes. -
Alternative names
- RNA polymerase sigma 32 factor antibody
- rpo H antibody
- rpoH antibody
Images
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Western blot - Anti-Sigma 32 antibody (ab26890)Lane 1 : Pre-bleed at 1/2000 dilution
Lane 2 : Anti-serum at 1/2000 dilution
All lanes : Antigen
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : anti-rabbit IgG-HRP
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Mahalakshmi S et al. yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli. Mol Microbiol 91:145-57 (2014). Read more (PubMed: 24266962) »
