Overview

  • Product name

    Anti-Sigma 32 antibody
  • Description

    Rabbit polyclonal to Sigma 32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length Sigma 32 protein (Serratia marcescens)

Properties

Applications

Our Abpromise guarantee covers the use of ab26890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

Target

  • Relevance

    When cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes.
  • Alternative names

    • RNA polymerase sigma 32 factor antibody
    • rpo H antibody
    • rpoH antibody

Images

  • Lane 1 : Pre-bleed at 1/2000 dilution
    Lane 2 : Anti-serum at 1/2000 dilution

    All lanes : Antigen

    Lysates/proteins at 4 µg per lane.

    Secondary
    All lanes : anti-rabbit IgG-HRP

    Developed using the ECL technique.

    Predicted band size: 32 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Mahalakshmi S  et al. yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli. Mol Microbiol 91:145-57 (2014). Read more (PubMed: 24266962) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
10.9912 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Escherichia coli Cell lysate - whole cell (no specification)
Specification
no specification
Treatment
100 ´M Nalidixic Acid for 18 hours
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

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Verified customer

Submitted Feb 18 2015

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