Key features and details
- Rabbit polyclonal to Sigma1-receptor
- Suitable for: WB, IHC-P
- Reacts with: Human, Zebrafish
- Isotype: IgG
Product nameAnti-Sigma1-receptor antibody
See all Sigma1-receptor primary antibodies
DescriptionRabbit polyclonal to Sigma1-receptor
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Rhesus monkey
Recombinant fragment corresponding to Human Sigma1-receptor aa 8-223.
Database link: Q99720
- 293T whole cell lysate; Human SW480 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFunctions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration.
Tissue specificityWidely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells.
Involvement in diseaseAmyotrophic lateral sclerosis 16, juvenile
Sequence similaritiesBelongs to the ERG2 family.
Cellular localizationNucleus inner membrane. Nucleus outer membrane. Endoplasmic reticulum membrane. Lipid droplet. Cell junction. Cell membrane. Cell projection > growth cone. Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum. Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding.
- Information by UniProt
- Aging associated gene 8 protein antibody
- Aging-associated gene 8 protein antibody
- AL024364 antibody
All lanes : Anti-Sigma1-receptor antibody (ab151288) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : Sigma1-receptor Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 25 kDa
Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft labeling Sigma1-receptor with ab151288 at 1/100 dilution.
Anti-Sigma1-receptor antibody (ab151288) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS PAGE
ab151288 has been referenced in 1 publication.
- Velazquez R et al. Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activation. Aging Cell 18:e13037 (2019). PubMed: 31560162