Western blot abreview for Anti-OPRS1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Cerebral)
Loading amount
50 µg
Specification
Cerebral
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~25 kDa Non-specific: ~75, ~250 kDa

Abcam user community

Verified customer

Submitted Aug 31 2010

Sign up