Western blot abreview for Anti-Sigma1-receptor antibody

Below Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y cells)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
60 µg
Specification
SH-SY5Y cells
Blocking step
BlockingOne as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Sigma A3687-.1ML
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase

Detection

Detection method
NBT/BCIP
Exposure
3 hour(s), 0 minute(s) and 0 second(s)

Additional data

Abcam response
Thank you for having tested ab53852 in an untested species (human). We recommend denaturing and reducing conditions to make this antibody work in western blot. We welcome any additional feedbacks for ab53852 in human samples with participation to our AbTrial program: www.abcam.com/abtrial

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Verified customer

Submitted Aug 04 2017

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