Key features and details
- Rabbit polyclonal to Signal peptide peptidase-like 2B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Signal peptide peptidase-like 2B antibody
DescriptionRabbit polyclonal to Signal peptide peptidase-like 2B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Signal peptide peptidase-like 2B aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in WI38 Whole Cell Lysate, Human Thymus Tissue Lysate and Human Brain Tissue Lysate (data not shown).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab43856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).|
RelevanceThe SPPLs are a group of aspartic proteases that are responsible for proteolysis of type II membrane-anchored signalling factors. SPPL2b, unlike other SPPL family members is found in endosomes/lysosomes, and not the endoplasmic reticulum, and has a role in catalysing intramembrane cleavage of tumour necrosis factor alpha (TNFalpha).
Cellular localizationMembrane; multi-pass membrane protein of endosomes/lysosomes.
- IMP4 antibody
- Intramembrane protease 4 antibody
- Protein SPP-like 2B antibody
- SPPL2B antibody
All lanes : Anti-Signal peptide peptidase-like 2B antibody (ab43856) at 1 µg/ml
Lane 1 :
WI-38 whole cell lysate (ab3960)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4) of Signal peptide peptidase-like 2B. The predicted molecular weights of isoforms (1,2,3,4) are 65 kDa, 35 kDa, 34 kDa and 56 kDa respectively. The band observed at 37 kDa may represent isoform 2 of Signal peptide peptidase-like 2B.
ab43856 has not yet been referenced specifically in any publications.