Overview

  • Product name

    Anti-Signal peptide peptidase-like 2B antibody
  • Description

    Rabbit polyclonal to Signal peptide peptidase-like 2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Signal peptide peptidase-like 2B aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab43855)

  • Positive control

    • This antibody gave a positive signal in WI38 Whole Cell Lysate, Human Thymus Tissue Lysate and Human Brain Tissue Lysate (data not shown).

Properties

Applications

Our Abpromise guarantee covers the use of ab43856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).

Target

  • Relevance

    The SPPLs are a group of aspartic proteases that are responsible for proteolysis of type II membrane-anchored signalling factors. SPPL2b, unlike other SPPL family members is found in endosomes/lysosomes, and not the endoplasmic reticulum, and has a role in catalysing intramembrane cleavage of tumour necrosis factor alpha (TNFalpha).
  • Cellular localization

    Membrane; multi-pass membrane protein of endosomes/lysosomes.
  • Database links

  • Alternative names

    • IMP4 antibody
    • Intramembrane protease 4 antibody
    • Protein SPP-like 2B antibody
    • SPPL2B antibody

Images

  • All lanes : Anti-Signal peptide peptidase-like 2B antibody (ab43856) at 1 µg/ml

    Lane 1 : WI-38 whole cell lysate (ab3960)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4) of Signal peptide peptidase-like 2B.  The predicted molecular weights of isoforms (1,2,3,4) are 65 kDa, 35 kDa, 34 kDa and 56 kDa respectively. The band observed at 37 kDa may represent isoform 2 of Signal peptide peptidase-like 2B.

References

ab43856 has not yet been referenced specifically in any publications.

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