Product nameAnti-Signal peptide peptidase-like 2B antibody
DescriptionRabbit polyclonal to Signal peptide peptidase-like 2B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- This antibody gave a positive signal in WI38 Whole Cell Lysate, Human Thymus Tissue Lysate and Human Brain Tissue Lysate (data not shown).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab43856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).|
RelevanceThe SPPLs are a group of aspartic proteases that are responsible for proteolysis of type II membrane-anchored signalling factors. SPPL2b, unlike other SPPL family members is found in endosomes/lysosomes, and not the endoplasmic reticulum, and has a role in catalysing intramembrane cleavage of tumour necrosis factor alpha (TNFalpha).
Cellular localizationMembrane; multi-pass membrane protein of endosomes/lysosomes.
- IMP4 antibody
- Intramembrane protease 4 antibody
- Protein SPP-like 2B antibody
- SPPL2B antibody
All lanes : Anti-Signal peptide peptidase-like 2B antibody (ab43856) at 1 µg/ml
Lane 1 :
WI-38 whole cell lysate (ab3960)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4) of Signal peptide peptidase-like 2B. The predicted molecular weights of isoforms (1,2,3,4) are 65 kDa, 35 kDa, 34 kDa and 56 kDa respectively. The band observed at 37 kDa may represent isoform 2 of Signal peptide peptidase-like 2B.
ab43856 has not yet been referenced specifically in any publications.