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    sik1-antibody-ab217809.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Other
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Anti-SIK1 antibody (ab217809)

  • Datasheet
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Western blot - Anti-SIK1 antibody (ab217809)

    Key features and details

    • Rabbit polyclonal to SIK1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human SIK1 protein (ab195112)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    IHC
    DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) (ab210060)

    View more associated products

    Overview

    • Product name

      Anti-SIK1 antibody
    • Description

      Rabbit polyclonal to SIK1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Human
    • Immunogen

      Synthetic peptide within Human SIK1 aa 40-90 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
      Sequence:

      VVKLARHRVTK TQVAIKIIDK TRLDSSNLEK IYREVQLMKL LNHPHIIKLY


      Database link: P57059
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: Mouse adrenal lysate.
    • General notes

      This product was previously labelled as Snf1lk/SIK-1

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: 50% Glycerol, 1% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Kinases/Phosphatases
      • Other
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Acetylation
      • HDACs
      • Class II / Hda1 Class
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Cardiomyocyte precursors
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Myocardial regeneration

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Mouse adrenal tissue lysate - total protein (ab4019)
    • Recombinant Protein

      • Recombinant human SIK1 protein (ab195112)

    Applications

    Our Abpromise guarantee covers the use of ab217809 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/100 - 1/1000. Predicted molecular weight: 85 kDa.

    Target

    • Function

      Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
      Contains 1 protein kinase domain.
      Contains 1 UBA domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus. Translocates to the cytoplasm on phosphorylation where it binds binding to YWHAZ.
    • Target information above from: UniProt accession P57059 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 150094 Human
      • Entrez Gene: 17691 Mouse
      • Omim: 605705 Human
      • SwissProt: P57059 Human
      • SwissProt: Q60670 Mouse
      • Unigene: 282113 Human
      • Unigene: 700423 Human
      • Unigene: 290941 Mouse
      • Alternative names

        • KID2 antibody
        • MSK antibody
        • myocardial SNF1 like kinase antibody
        • QIK antibody
        • Qin-induced kinase antibody
        • Salt inducible kinase 1 antibody
        • Salt-inducible protein kinase 1 antibody
        • Serine threonine protein kinase SNF1 like kinase 1 antibody
        • Serine threonine protein kinase SNF1LK antibody
        • Serine/threonine-protein kinase SIK1 antibody
        • Serine/threonine-protein kinase SNF1-like kinase 1 antibody
        • Serine/threonine-protein kinase SNF1LK antibody
        • SIK-1 antibody
        • SIK1 antibody
        • SIK1_HUMAN antibody
        • SNF1 like kinase antibody
        • Snf1lk antibody
        see all

      Images

      • Western blot - Anti-SIK1 antibody (ab217809)
        Western blot - Anti-SIK1 antibody (ab217809)
        Anti-SIK1 antibody (ab217809) at 1/300 dilution + mouse adrenal lysate

        Secondary
        Goat anti-rabbit IgG antibody (H+L), HRP at 1/5000 dilution

        Predicted band size: 85 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab217809? Please let us know so that we can cite the reference in this datasheet.

      ab217809 has been referenced in 1 publication.

      • Hong B  et al. Activation of the LKB1-SIK1 signaling pathway inhibits the TGF-ß-mediated epithelial-mesenchymal transition and apoptosis resistance of ovarian carcinoma cells. Mol Med Rep 17:2837-2844 (2018). PubMed: 29257268

      Customer reviews and Q&As

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