Product nameAnti-SIK2 antibody
See all SIK2 primary antibodies
DescriptionRabbit polyclonal to SIK2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human, Chimpanzee
Synthetic peptide within Human SIK2 aa 100-150. The exact sequence is proprietary.
Database link: NP_056006
This product was previously labelled as Snf1lk2, Snf1lk+Snf1lk2+SIK3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120, 25 kDa (predicted molecular weight: 104 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPhosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
- Information by UniProt
- DKFZp434K1115 antibody
- EC 18.104.22.168 antibody
- KIAA0781 antibody
All lanes : Anti-SIK2 antibody (ab53423) at 2 µg/ml
Lane 1 : Human brain
Lane 2 : Human brain with immunizing peptide
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
NB While the predicted molecular weight is 104kDa, both the bands at 120kDa and at 25kDa are blocked by immunizing peptide.
IHC image of ab53423 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53423, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Weber GJ et al. Transcriptome alterations following developmental atrazine exposure in zebrafish are associated with disruption of neuroendocrine and reproductive system function, cell cycle, and carcinogenesis. Toxicol Sci 132:458-66 (2013). Read more (PubMed: 23358194) »