Key features and details
- Mouse monoclonal [S15G10] to SIK2
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SIK2 antibody [S15G10]
See all SIK2 primary antibodies
DescriptionMouse monoclonal [S15G10] to SIK2
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human SIK2. (BAA34501.3)
- IHC-P: Human Pancreas tissue; WB: HeLa; ICC/IF: Nocodazole treated HeLa;
This product was previously labelled as Snf1lk2, Snf1lk+Snf1lk2+SIK3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab115567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Predicted molecular weight: 104 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/400.|
FunctionPhosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
- Information by UniProt
- DKFZp434K1115 antibody
- EC 22.214.171.124 antibody
- KIAA0781 antibody
Anti-SIK2 antibody [S15G10] (ab115567) + HeLa cell lysate
Predicted band size: 104 kDa
Overnight nocodazole treated HeLa cells stained with purified ab115567, followed by Cy3 conjugated Goat anti-mouse IgG and DAPI.
ab115567, at 10µg/ml, staining SIK2 in Formalin-fixed, Paraffin-embedded Human Pancreas tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115567 has not yet been referenced specifically in any publications.