Overview

  • Product name

    Anti-SIK3 antibody - C-terminal
    See all SIK3 primary antibodies
  • Description

    Chicken polyclonal to SIK3 - C-terminal
  • Host species

    Chicken
  • Specificity

    At least three isoforms of SIK3 are known to exist and ab211424 will detect two isoforms. ab211424 is predicted to not cross-react with SIK1 and SIK2.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human SIK3 (C terminal). 18 amino acid peptide.
    Database link: Q9Y2K2

  • Positive control

    • Human breast tissue; Rat brain cells; Rat brain tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab211424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.

Target

  • Relevance

    Activated by phosphorylation on Thr 163 by STK11 in complex with STE20 related adapter alpha (STRAD alpha) pseudo kinase and CAB39. Binds to and is activated by YWHAZ when phosphorylated on Thr 163.
  • Cellular localization

    Cytoplasm
  • Database links

  • Alternative names

    • FLJ12240 antibody
    • KIAA0999 antibody
    • L19 antibody
    • QSK antibody
    • Salt inducible kinase 3 antibody
    • serine/threonine-protein kinase QSK antibody
    • Serine/threonine-protein kinase SIK3 antibody
    • SIK 3 antibody
    • SIK family kinase 3 antibody
    • SIK3_HUMAN antibody
    see all

Images

  • All lanes : Anti-SIK3 antibody - C-terminal (ab211424) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate with blocking peptide

    Predicted band size: 140 kDa

  • Immunofluorescenct analysis of Rat brain cells labeling SIK3 with ab211424 at 20µg/ml.

  • Immunohistochemical analysis of rormalin fixed, paraffin-embedded Human breast tissue labeling SIK3 with ab211424 at 5µg/ml.

References

This product has been referenced in:

  • Alotaibi D  et al. Potential anticancer effect of prostratin through SIK3 inhibition. Oncol Lett 15:3252-3258 (2018). Read more (PubMed: 29435066) »
  • Amara S  et al. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS One 12:e0180097 (2017). Read more (PubMed: 28658303) »
See all 2 Publications for this product

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