Key features and details
- Chicken polyclonal to SIK3 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgY
Product nameAnti-SIK3 antibody - C-terminal
See all SIK3 primary antibodies
DescriptionChicken polyclonal to SIK3 - C-terminal
SpecificityAt least three isoforms of SIK3 are known to exist and ab211424 will detect two isoforms. ab211424 is predicted to not cross-react with SIK1 and SIK2.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human SIK3 (C terminal). 18 amino acid peptide.
Database link: Q9Y2K2
- Human breast tissue; Rat brain cells; Rat brain tissue lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.|
RelevanceActivated by phosphorylation on Thr 163 by STK11 in complex with STE20 related adapter alpha (STRAD alpha) pseudo kinase and CAB39. Binds to and is activated by YWHAZ when phosphorylated on Thr 163.
- FLJ12240 antibody
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All lanes : Anti-SIK3 antibody - C-terminal (ab211424) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate with blocking peptide
Predicted band size: 140 kDa
Immunofluorescenct analysis of Rat brain cells labeling SIK3 with ab211424 at 20µg/ml.
Immunohistochemical analysis of rormalin fixed, paraffin-embedded Human breast tissue labeling SIK3 with ab211424 at 5µg/ml.
ab211424 has been referenced in 2 publications.
- Alotaibi D et al. Potential anticancer effect of prostratin through SIK3 inhibition. Oncol Lett 15:3252-3258 (2018). PubMed: 29435066
- Amara S et al. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS One 12:e0180097 (2017). PubMed: 28658303