Product nameAnti-SIK3 antibody - C-terminal
See all SIK3 primary antibodies
DescriptionChicken polyclonal to SIK3 - C-terminal
SpecificityAt least three isoforms of SIK3 are known to exist and ab211424 will detect two isoforms. ab211424 is predicted to not cross-react with SIK1 and SIK2.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human SIK3 (C terminal). 18 amino acid peptide.
Database link: Q9Y2K2
- Human breast tissue; Rat brain cells; Rat brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.|
RelevanceActivated by phosphorylation on Thr 163 by STK11 in complex with STE20 related adapter alpha (STRAD alpha) pseudo kinase and CAB39. Binds to and is activated by YWHAZ when phosphorylated on Thr 163.
- FLJ12240 antibody
- KIAA0999 antibody
- L19 antibody
All lanes : Anti-SIK3 antibody - C-terminal (ab211424) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate with blocking peptide
Predicted band size: 140 kDa
Immunofluorescenct analysis of Rat brain cells labeling SIK3 with ab211424 at 20µg/ml.
Immunohistochemical analysis of rormalin fixed, paraffin-embedded Human breast tissue labeling SIK3 with ab211424 at 5µg/ml.
This product has been referenced in:
- Alotaibi D et al. Potential anticancer effect of prostratin through SIK3 inhibition. Oncol Lett 15:3252-3258 (2018). Read more (PubMed: 29435066) »
- Amara S et al. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS One 12:e0180097 (2017). Read more (PubMed: 28658303) »