Recombinant Anti-SIK3 antibody [EPR20880-249] (ab227044)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20880-249] to SIK3
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SIK3 antibody [EPR20880-249]
See all SIK3 primary antibodies -
Description
Rabbit monoclonal [EPR20880-249] to SIK3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: N41 transfected with scrambled siRNA control, SIK3 knockdown N41 and Neuro-2a whole cell lysate; Mouse hippocampus, Rat brain, Rat hippocampus, Human hippocampus, Rat liver and Mouse cortex tissue lysate. ICC/IF: Neuro-2a cells. Flow Cyt: Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20880-249 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 140 kDa.
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ICC/IF |
1/50.
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Flow Cyt |
1/500.
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Notes |
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WB
1/1000. Predicted molecular weight: 140 kDa. |
ICC/IF
1/50. |
Flow Cyt
1/500. |
Target
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Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Post-translational
modificationsPhosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. -
Cellular localization
Cytoplasm. Locates to punctate structures within the cytoplasm on binding to YWHAZ. - Information by UniProt
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Database links
- Entrez Gene: 23387 Human
- Entrez Gene: 70661 Mouse
- Entrez Gene: 684112 Rat
- Omim: 614776 Human
- SwissProt: Q9Y2K2 Human
- SwissProt: Q6P4S6 Mouse
- Unigene: 167451 Human
- Unigene: 219459 Mouse
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Alternative names
- FLJ12240 antibody
- KIAA0999 antibody
- L19 antibody
see all
Images
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All lanes : Anti-SIK3 antibody [EPR20880-249] (ab227044) at 1/1000 dilution
Lane 1 : N41 (Embryonic Mouse Hypothalamus Cell Line) transfected with scrambled siRNA control whole cell lysate
Lane 2 : SIK3 knockdown N41 whole cell lysate
Lane 3 : Mouse hippocampus tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 140 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The control and SIK3 knockdown cell lysates were kindly provided by an anonymous collaborator.
Exposure time: Lanes 1-3: 26 seconds; Lane 4: 15 seconds.
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All lanes : Anti-SIK3 antibody [EPR20880-249] (ab227044) at 1/1000 dilution
Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lanes 2-4: 8 seconds.
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Anti-SIK3 antibody [EPR20880-249] (ab227044) at 0.789 µg/ml + Mouse cortex tissue lysate at 4 µg
Predicted band size: 140 kDa
Observed band size: 150,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Data was kindly provided by Dr. Qinghua Liu, NIBS.
The 1st Ab working concentration was 5 ug/ml.
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Immunocytochemical analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling SIK3 with ab227044 at 1/50 (15.78 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cells. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling SIK3 with ab227044 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab227044 has not yet been referenced specifically in any publications.