Key features and details
- Rabbit polyclonal to SIKE1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SIKE1 antibody
See all SIKE1 primary antibodies
DescriptionRabbit polyclonal to SIKE1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human rectum tissue; Human liver and tonsil lysates; RT 4 and U 251 MG cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionPhysiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
Tissue specificityWidely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).
Sequence similaritiesBelongs to the SIKE family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
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Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
ab121860, at 1/150 dilution, staining SIKE1 in glandular cells of paraffin-embedded Human rectum tissue by Immunohistochemistry.
All lanes : Anti-SIKE1 antibody (ab121860) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab121860 has been referenced in 3 publications.
- Bae SJ et al. STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism. Elife 9:N/A (2020). PubMed: 32292165
- Tang Y et al. Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling. Cell Discov 5:3 (2019). PubMed: 30622739
- Bae SJ et al. SAV1 promotes Hippo kinase activation through antagonizing the PP2A phosphatase STRIPAK. Elife 6:N/A (2017). PubMed: 29063833