Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SIKE1 antibody [EPR14692] (ab183509)

Overview

  • Product name

    Anti-SIKE1 antibody [EPR14692]
    See all SIKE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14692] to SIKE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SIKE1 aa 1-150. The exact sequence is proprietary.
    Database link: Q9BRV8

  • Positive control

    • SH-SY5Y, HeLa and 293 cell lysates; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14692
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30 - 1/50.
IHC-P 1/50.
WB 1/10000 - 1/50000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Target

  • Function

    Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
  • Tissue specificity

    Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).
  • Sequence similarities

    Belongs to the SIKE family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ21168 antibody
    • RGD1311316 antibody
    • RP5-1000E10.4 antibody
    • SIKE antibody
    • sike1 antibody
    • SIKE1_HUMAN antibody
    • Suppressor of IKBKE 1 antibody
    • Suppressor of IKK-epsilon antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SIKE1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: PC3 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab183509 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab183509 was shown to specifically react with SIKE1 when SIKE1 knockout samples were used. Wild-type and SIKE1 knockout samples were subjected to SDS-PAGE. ab183509 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-SIKE1 antibody [EPR14692] (ab183509) at 1/20000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa
    Additional bands at: 24 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling SIKE1 with ab183509 at 1/50.

  • Lysate from 293 cells was immunoprecipitated with ab183509 at a 1/50 dilution. For the subsequent blot, ab183509 used at a 1/10000 dilution with an anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Lane 2: TBS instead of lysate.

References

ab183509 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183509.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up