The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 52 kDa.
Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.
Involvement in disease
Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS.
Belongs to the SIL1 family.
Expressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labeling SIL1 with ab175981 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometry - Anti-SIL1 antibody (ab175981)
Flow cytometric analysis of HepG2 cells labeling SIL1 with ab175981 at 1/10 (lower histogram) compared to a negative control cell (upper histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.