Overview

  • Product name

  • Description

    Rabbit polyclonal to SIL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human SIL1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H173

  • Positive control

    • IHC-P: MCF7 xenograft. Rat muscle tissue. WB: A431 whole cell lysate. Rat kidney tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.

Target

  • Function

    Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
  • Tissue specificity

    Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.
  • Involvement in disease

    Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS.
  • Sequence similarities

    Belongs to the SIL1 family.
  • Developmental stage

    Expressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAP antibody
    • BiP associated protein antibody
    • BiP-associated protein antibody
    • Endoplasmic reticulum chaperone SIL 1 antibody
    • Endoplasmic reticulum chaperone SIL1 antibody
    • MSS antibody
    • Nucleotide exchange factor SIL 1 antibody
    • Nucleotide exchange factor SIL1 antibody
    • SIL 1 antibody
    • sil1 antibody
    • SIL1 homolog antibody
    • SIL1 homolog endoplasmic reticulum chaperone (S. cerevisiae) antibody
    • SIL1 homolog endoplasmic reticulum chaperone antibody
    • SIL1_HUMAN antibody
    • UGL 5 antibody
    • ULG5 antibody
    see all

Images

  • Paraffin-embedded rat muscle tissue stained for SIL1 with ab228844 at a 1/500 dilution in immunohistochemical analysis.

  • Anti-SIL1 antibody (ab228844) at 1/5000 dilution + Rat kidney tissue extract at 50 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa



    10% SDS-PAGE

  • Anti-SIL1 antibody (ab228844) at 1/5000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa



    10% SDS-PAGE

  • Paraffin-embedded MCF7 xenograft stained for SIL1 with ab228844 at a 1/250 dilution in immunohistochemical analysis.

References

ab228844 has not yet been referenced specifically in any publications.

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