Anti-SIL1 antibody (ab228868)
Key features and details
- Rabbit polyclonal to SIL1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SIL1 antibody
See all SIL1 primary antibodies -
Description
Rabbit polyclonal to SIL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Chimpanzee -
Immunogen
Recombinant fragment within Human SIL1 (internal sequence). The exact sequence is proprietary.
Database link: Q9H173 -
Positive control
- WB: Rat liver tissue extract; A431 whole cell lysate. IHC-P: Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
Target
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Function
Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5. -
Tissue specificity
Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain. -
Involvement in disease
Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS. -
Sequence similarities
Belongs to the SIL1 family. -
Developmental stage
Expressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 747459 Chimpanzee
- Entrez Gene: 520650 Cow
- Entrez Gene: 64374 Human
- Entrez Gene: 81500 Mouse
- Entrez Gene: 291673 Rat
- Omim: 608005 Human
- SwissProt: Q9H173 Human
- SwissProt: Q9EPK6 Mouse
see all -
Alternative names
- BAP antibody
- BiP associated protein antibody
- BiP-associated protein antibody
see all
Images
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Anti-SIL1 antibody (ab228868) at 1/5000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 52 kDa10% SDS-PAGE gel.
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Anti-SIL1 antibody (ab228868) at 1/5000 dilution + Rat liver tissue extract at 50 µg
Predicted band size: 52 kDa10% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tisue stained for SIL1 using ab228868 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228868 has not yet been referenced specifically in any publications.