Overview

  • Product name
    Anti-SIL1 antibody - C-terminal
    See all SIL1 primary antibodies
  • Description
    Rabbit polyclonal to SIL1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 398-461 of Human SIL1 (UniProt: Q9H173).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human TW2.6 xenograft tissue.
  • General notes
    Store as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8.

Target

  • Function
    Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
  • Tissue specificity
    Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.
  • Involvement in disease
    Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS.
  • Sequence similarities
    Belongs to the SIL1 family.
  • Developmental stage
    Expressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAP antibody
    • BiP associated protein antibody
    • BiP-associated protein antibody
    • Endoplasmic reticulum chaperone SIL 1 antibody
    • Endoplasmic reticulum chaperone SIL1 antibody
    • MSS antibody
    • Nucleotide exchange factor SIL 1 antibody
    • Nucleotide exchange factor SIL1 antibody
    • SIL 1 antibody
    • sil1 antibody
    • SIL1 homolog antibody
    • SIL1 homolog endoplasmic reticulum chaperone (S. cerevisiae) antibody
    • SIL1 homolog endoplasmic reticulum chaperone antibody
    • SIL1_HUMAN antibody
    • UGL 5 antibody
    • ULG5 antibody
    see all

Images

  • All lanes : Anti-SIL1 antibody - C-terminal (ab155121) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human TW2.6 xenograft tissue, labeling SIL1 with ab155121 at 1/100 dilution.

References

ab155121 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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