Product nameAnti-Silencer of Death Domain antibody [EPR3596]
See all Silencer of Death Domain primary antibodies
DescriptionRabbit monoclonal [EPR3596] to Silencer of Death Domain
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human Silencer of Death Domain (UniProt O95429).
- WB: HeLa, HepG2, and Molt-4 cell lysates ICC/IF: HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 50 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling.
Sequence similaritiesContains 1 BAG domain.
- Information by UniProt
- BAG 4 antibody
- BAG family molecular chaperone regulator 4 antibody
- BAG-4 antibody
All lanes : Anti-Silencer of Death Domain antibody [EPR3596] (ab108988) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Molt-4 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Immunofluorescent staining of Silencer of Death Domain in HeLa cells, using ab108988 at a 1/100 dilution.
ab108988 has not yet been referenced specifically in any publications.