Product nameSilver Enhancer Kit (Light Insensitive)
Sample typeTissue, Suspension cells
Abcam’s Silver Enhancer (Light Insensitive) Kit (ab170733) is a convenient, light-insensitive silver enhancement system for use with gold particle immunoreagents, which may be used for light microscopy or to visualize gold particles on gels or blots. Silver Enhancer (Light Insensitive) is nucleated quickly by gold nanoparticles, resulting in the precipitation of metallic silver and the formation of a dark brown to black signal. The system has markedly delayed self-nucleation resulting in high contrast and very low background and non-specific staining. ab170733 consists of 125 mL ready-to-use enhancer and 125 mL ready-to-use initiator; the reagent is formed by combining equal volumes of these solutions. The mixture is usable only for a defined time period, as given below, and should therefore be prepared immediately before use. For electron microscopy, we recommend ab170732.
Tested applicationsSuitable for: WB, Electron Microscopy, IHC, ICCmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 ml Enhancer 1 x 125ml Initiator 1 x 125ml
Our Abpromise guarantee covers the use of ab170733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|Electron Microscopy||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
ab170733 has not yet been referenced specifically in any publications.