Overview

  • Product name

    Anti-SIM2 antibody [EPR7878]
    See all SIM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7878] to SIM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SIM2 (Q14190)

  • Positive control

    • HepG2 whole cell lysate (ab7900); Human fetal forebrain and Human fetal brain lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 73 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.
      Contains 1 Single-minded C-terminal domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • bHLHe15 antibody
      • Class E basic helix loop helix protein 15 antibody
      • Class E basic helix-loop-helix protein 15 antibody
      • MGC119447 antibody
      • SIM 2 antibody
      • SIM antibody
      • Sim2 antibody
      • SIM2_HUMAN antibody
      • Single minded homolog 2 (Drosophila) antibody
      • Single minded homolog 2 antibody
      • Single-minded homolog 2 antibody
      • Transcription factor SIM2 antibody
      see all

    Images

    • Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 73 kDa
      Observed band size: 64 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution

      Lane 1 : Human fetal forebrain lysate
      Lane 2 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 73 kDa
      Observed band size: 64 kDa why is the actual band size different from the predicted?

    References

    ab131161 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    For ab94630 DISCOUNT CODE: XXXXXXXXXXX
    Expiration date: 27-10-2012

    For ab131161 DISCOUNT CODE: XXXXXXXXX
    Expiration date: 27-10-2012

    I am very pleased to hear you would like to accept our offer and test ab131161 and ab94630in [IP]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [IP] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    PS: The mouse non reactivity is indeed based on tested data.

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