Recombinant Anti-SIM2 antibody [EPR7878] (ab131161)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7878] to SIM2
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-SIM2 antibody [EPR7878]
See all SIM2 primary antibodies -
Description
Rabbit monoclonal [EPR7878] to SIM2 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide, corresponding to residues in Human SIM2 (Q14190)
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Positive control
- HepG2 whole cell lysate (ab7900); Human fetal forebrain and Human fetal brain lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7878 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 73 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 73 kDa). |
Target
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Function
Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 Single-minded C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6493 Human
- Omim: 600892 Human
- SwissProt: Q14190 Human
- Unigene: 146186 Human
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Alternative names
- bHLHe15 antibody
- Class E basic helix loop helix protein 15 antibody
- Class E basic helix-loop-helix protein 15 antibody
see all
Images
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Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted? -
All lanes : Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution
Lane 1 : Human fetal forebrain lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (0)
ab131161 has not yet been referenced specifically in any publications.