Key features and details
- One-wash 90 minute protocol
Product nameSimpleStep ELISA® Custom ELISA Buffer Pack
SimpleStep ELISA® Custom ELISA Kit (ab270552) is the latest cutting-edge ELISA kit with a built-in system for antibody labeling, enabling the rapid and easy development of in-house ELISAs using any antibody pair, while eliminating the need to perform plate coating procedures.
It is powered by the combination of a proprietary technologies, enabling you to reduce the amount of capture antibody required, measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.
The capture antibody is quickly and easily conjugated with the peptide supplied within the kit. Once added to the plate, the interaction between the antibody and the peptide immobilizes the immune-complex to the plate.
The detection antibody is similarly conjugated with HRP using the HRP conjugation reagent, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.
SimpleStep ELISA® Custom ELISA Buffer Pack (ab270697) is the buffer pack available separately.
This product is manufactured by Expedeon, an Abcam company. It was previously called CaptSure™ ELISA Buffer Pack (5x96well) . Expedeon product code 6300008 is the same as the 5 x 96 test size of this product.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 5 x 96 tests Detection Reagent (ab270697) 1 unit HRP-Labeled Antibody Dilution Buffer (ab270697) 1 unit Lysis Extraction Buffer (ab270697) 1 unit Peptide tagged Antibody Dilution Buffer (ab270697) 1 unit Sample Dilution Buffer (ab270697) 2 units Stop Solution (ab270697) 1 unit Wash Buffer (ab270697) 1 unit
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270697 has not yet been referenced specifically in any publications.