Product nameSimpleStep ELISA® Custom ELISA Kit
SimpleStep ELISA® Custom ELISA Kit (ab270552) is the latest cutting-edge ELISA kit with a built-in system for antibody labeling, enabling the rapid and easy development of in-house ELISAs using any antibody pair, while eliminating the need to perform plate coating procedures.
It is powered by the combination of a proprietary technologies, enabling you to reduce the amount of capture antibody required, measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.
The capture antibody is quickly and easily conjugated with the peptide supplied within the kit. Once added to the plate, the interaction between the antibody and the peptide immobilizes the immune-complex to the plate.
The detection antibody is similarly conjugated with HRP using the HRP conjugation reagent, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.
This kit has ready-to-go assay plates, pre-coated with antibody, which are specific for the peptide.
This product is manufactured by Expedeon, an Abcam company. It was previously called CaptSure DIY ELISA Kit (kit sufficient for 5 x 96 well plates). Expedeon product code 6300020 is the same as the 5 x 96 tests size of this product.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsPlease refer to protocols.
Components 5 x 96 tests Assay Plates 5 units Detection Reagent 1 unit HRP-Labeled Antibody Dilution Buffer 1 unit HRP Mix 1 vial Capture Modifier reagent 1 vial Capture Quencher reagent 1 vial Capture Peptide 3 vials HRP-modifier reagent 1 vial HRP-quencher reagent 1 vial Lysis Extraction Buffer 1 unit Peptide tagged Antibody Dilution Buffer 1 unit Sample Dilution Buffer 2 units Stop Solution 1 unit Wash Buffer 1 unit
Our Abpromise guarantee covers the use of ab270552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
SimpleStep ELISA® Custom ELISA Kit and conventional passive coating approach were compared. With ab270552, 10x less capture antibody can be used without affecting the assay performance.
Other solution-phase binding ELISA formats typically use biotin to immobilize immunocomplexes. These can constrain the sample types that can be used with the assay. In contrast, ab270552 has been specifically designed to be used with most sample matrices without interference.
ab270552 has not yet been referenced specifically in any publications.