Sinomenine hydrochloride, Morphinan analog (ab141190)
Key features and details
- Natural anti-inflammatory morphinan analog; potently releases histamine
- CAS Number: 6080-33-7
- Purity: > 99%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Sinomenium acutum
Overview
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Product name
Sinomenine hydrochloride, Morphinan analog -
Description
Natural anti-inflammatory morphinan analog; potently releases histamine -
Biological description
Natural anti-inflammatory morphinan analog, able to weakly bind to the μ-opioid receptor. Potently releases histamine following mast cell degranulation. Inhibits oligomeric amyloid-β-induced increases in ROS, NO and other inflammatory molecules. Stimulates acute renewal of human embryonic stem cells (ESCs) in vitro.
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Purity
> 99% -
CAS Number
6080-33-7 -
Chemical structure
Properties
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Chemical name
(9α,13α,14α)-7,8-Didehydro-4-hydroxy-3,7-dimethoxy-17-methylmorphinan-6-one hydrochloride -
Molecular weight
365.86 -
Molecular formula
C19H23NO4.HCl -
PubChem identifier
5464452 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cl.COC3=C[C@@H]4C2Cc1ccc(OC)c(O)c1[C@]4(CCN2C)CC3=O -
Source
Sinomenium acutum
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
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Functional Studies - Sinomenine hydrochloride, Morphinan analog (ab141190)ab15191 staining COX2 in HepG2 cells treated with sinomenine hydrochloride (ab141190), by ICC/IF. Decrease of COX2 expression correlates with increased concentration of sinomenine hydrochloride, as described in literature.
The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141190 (sinomenine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15191 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab141190 has been referenced in 1 publication.
- Wang W et al. Sinomenine Attenuates Angiotensin II-Induced Autophagy via Inhibition of P47-Phox Translocation to the Membrane and Influences Reactive Oxygen Species Generation in Podocytes. Kidney Blood Press Res 41:158-67 (2016). PubMed: 26919366