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Rabbit polyclonal to SIPA1 - C-terminal
Synthetic peptide, corresponding to a region within C terminal amino acids 980-1042 of Human SIPA1 (UniProt: Q96FS4).
293T cell lysate, transfected with SIPA1; Mouse embryonic stem cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 112 kDa.
1/50 - 1/500.
GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Contains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain.
Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
Information by UniProt
GTPase activating protein Spa 1 antibody
GTPase-activating protein Spa-1 antibody
Western blot - Anti-SIPA1 antibody - C-terminal (ab155024)
All lanes : Anti-SIPA1 antibody - C-terminal (ab155024) at 1/500 dilution Lane 1 : 293T cell lysate, non-transfected Lane 2 : 293T cell lysate, transfected with SIPA1 Predicted band size: 112 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-SIPA1 antibody - C-terminal (ab155024)
Immunofluorescence analysis of methanol-fixed Mouse embryonic stem cells labeling SIPA1 with ab155024 at 1/200. Lower image shows co-staining with Hoechst 33342.
has not yet been referenced specifically in any publications.
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