Key features and details
- Rabbit polyclonal to SIPA1L1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-SIPA1L1 antibody
DescriptionRabbit polyclonal to SIPA1L1
Specificityab91586 is specific for SIPA1L1 and will not recognize the other SIPA1 family proteins.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to Human SIPA1L1 (N terminal). The antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SIPA1L1. It is located within amino acids 40 to 90.
Database link: O43166
- Rat and human brain tissue lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 200 kDa.|
RelevanceSIPA1L1 (Signal-induced proliferation associated-like protein 1) is a member of the SIPA1 family of RapGAPs. SIPA1L1 was initially identified as a binding partner and degradation target of the E6 oncoprotein of high-risk papillomaviruses. Recently, it was discovered that Casein kinase I epsilon (CKIe), a Wnt-regulated kinase that regulates Wnt/b-catenin signaling, also can bind to the carboxy-terminus of SIPA1L1. CKIe phosphorylates SIPA1L1, thereby reducing its stability and alleviating its inhibition of Rap1, a protein required for Wnt8/CKIe-mediated gastrulation during embryogenesis, suggesting SIPA1L1 plays important roles in embryo development as well as control of cell proliferation.
- DKFZp686G1344 antibody
- E6 targeted protein 1 antibody
- E6TP1 antibody
Immunofluorescence of SIPA1L1 in Human brain tissue with ab91586 at 20 ug/mL.
Immunohistochemistry of Human brain tissue with ab91586 at 5 μg/mL.
Lane 1 : Anti-SIPA1L1 antibody (ab91586) at 0.5 µg/ml
Lane 2 : Anti-SIPA1L1 antibody (ab91586) at 1 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 200 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91586 has not yet been referenced specifically in any publications.