Key features and details
- Rabbit polyclonal to SIRP alpha
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-SIRP alpha antibody
See all SIRP alpha primary antibodies
DescriptionRabbit polyclonal to SIRP alpha
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human SIRP alpha aa 105-324. Expressed in E.coli. N-terminal tags.
SIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVS GPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGES VSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPP TLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTE NKDGTYNWMSWLLVNVSAHR
Database link: P78324-1
- WB: Recombinant human SIRP alpha protein; HEK-293T cell lysate; Pig brain lysate. IHC-P: Human cerebrum, liver, breast cancer and stomach tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Sequence similaritiesContains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
- Information by UniProt
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-SIRP alpha antibody (ab232765) at 2 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig brain lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-SIRP alpha antibody (ab232765) at 2 µg + Recombinant human SIRP alpha protein
Predicted band size: 55 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232765 has not yet been referenced specifically in any publications.