- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-SIRP alpha antibody
See all SIRP alpha primary antibodies -
DescriptionMouse polyclonal to SIRP alpha
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Full length Human SIRP alpha protein (AAH33092.1)
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Positive control
- SIRP alpha transfected 293T cell lysate. Human liver tissue lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab77061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa. |
Target
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FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
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Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
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Sequence similaritiesContains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Images
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Western blot - Anti-SIRP alpha antibody (ab77061)All lanes : Anti-SIRP alpha antibody (ab77061) at 1 µg/ml
Lane 1 : SIRP alpha transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 56 kDa
Observed band size: 80 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-SIRP alpha antibody (ab77061)Anti-SIRP alpha antibody (ab77061) at 1 µg/ml + human liver tissue lysate at 25 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 56 kDa
Observed band size: 80 kDa (why is the actual band size different from the predicted?)
Protocols
References
ab77061 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"