Recombinant Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)
![Western blot - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)](https://www.abcam.com/ps/products/267/ab267410/Images/ab267410-395626-AntiSIRP-alpha-antibody-SADF101-western-blot-brain-u937-skmel28-human.jpg "Western blot - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [SADF10.1] to SIRP alpha - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free
See all SIRP alpha primary antibodies -
Description
Mouse monoclonal [SADF10.1] to SIRP alpha - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human brain tissue lysate. U937, SK-MEL-2 and SK-MEL-28 whole cell lysate. ICC/IF: THP-1 cells.
-
General notes
ab267410 is the carrier-free version of ab267409.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SADF10.1 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab267410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
-
Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Images
-
Western blot - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)All lanes : Anti-SIRP alpha antibody [SADF10.1] (ab267409) at 1.273 µg/ml
Lanes 1 & 3 : Human brain tissue lysate
Lane 2 : U937 (human histiocytic lymphoma monocyte), whole cell lysate
Lane 4 : SK-MEL-2 (human malignant melanoma), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 55 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile weight observed is consistent with what has been described in the literature (PMID: 9712903, 12773380).
Negative control: Jurkat (PMID: 17982459).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267409).
-
![Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)](https://www.abcam.com/ps/products/267/ab267410/Images/ab267410-395625-AntiSIRP-alpha-antibody-SADF101-immunocytochemistry-thp1-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling SIRP alpha with ab267409 at 127.3ug/ml, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab267409 at 127.3µg/ml followed by ab150080 at a 1/200 dilution.Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267409).
-
![Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)](https://www.abcam.com/ps/products/267/ab267410/Images/ab267410-3-benefits-of-recombinant-antibodies.png)
Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab267410 has been referenced in 1 publication.
- Lee WY et al. Novel structural determinants on SIRP alpha that mediate binding to CD47. J Immunol 179:7741-50 (2007). PubMed: 18025220
