Overview

  • Product name

  • Description

    Rabbit polyclonal to SIRPB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human SIRPB1 aa 30-371.
    Database link: O00241

  • Positive control

    • WB: A549, PC-3 and A575 whole cell lysates; Mouse heart tissue lysate. IHC-P: Human thyroid and colon cancer tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as SIRP beta 1/CD172b

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Relevance

    The protein encoded by the SIRPß1 gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins which are known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPß1 was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Cellular localization

    Cell Membrane; single-pass type I membrane protein
  • Database links

  • Alternative names

    • CD172 antigen like family member B antibody
    • CD172b antibody
    • CD172b antigen antibody
    • DKFZp686A05192 antibody
    • Signal regulatory protein beta 1 antibody
    • SIRP BETA 1 antibody
    • SIRP beta 1 isoform 3 antibody
    see all

Images

  • All lanes : Anti-SIRPB1 antibody (ab234681) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : A575 (human malignant melanoma cell line) whole cell lysate
    Lane 4 : Mouse heart lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 43 kDa

  • Paraffin-embedded human thyroid tissue stained for SIRPB1 using ab234681 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human colon cancer tissue stained for SIRPB1 using ab234681 at 1/100 dilution in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SIRPB1 (green) using ab234681 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

References

ab234681 has not yet been referenced specifically in any publications.

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