Key features and details
- Rabbit polyclonal to SIRPB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SIRPB1 antibody
See all SIRPB1 primary antibodies
DescriptionRabbit polyclonal to SIRPB1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human SIRPB1 aa 30-371.
Database link: O00241
- WB: A549, PC-3 and A575 whole cell lysates; Mouse heart tissue lysate. IHC-P: Human thyroid and colon cancer tissue. ICC/IF: HeLa cells.
This product was previously labelled as SIRP beta 1/CD172b
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab234681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
RelevanceThe protein encoded by the SIRPß1 gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins which are known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPß1 was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene.
Cellular localizationCell Membrane; single-pass type I membrane protein
- CD172 antigen like family member B antibody
- CD172b antibody
- CD172b antigen antibody
All lanes : Anti-SIRPB1 antibody (ab234681) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : A575 (human malignant melanoma cell line) whole cell lysate
Lane 4 : Mouse heart lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Paraffin-embedded human thyroid tissue stained for SIRPB1 using ab234681 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for SIRPB1 using ab234681 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SIRPB1 (green) using ab234681 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234681 has not yet been referenced specifically in any publications.