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Abcam’s SIRT2 Activity Assay Kit (Fluorometric) (ab156066) detects deacetylase activity of recombinant SIRT2.
Primarily, Abcam’s SIRT2 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT2 inhibitors or activators using recombinant SIRT2 or purified SIRT2.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT2.
2. Detecting the effects of pharmacological agents on SIRT2.
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.
|Developer||1 x 500µl|
|Fluoro-Deacetylated Peptide (0.2 mM)||1 x 100µl|
|Fluoro-Substrate Peptide (0.2 mM)||1 x 500µl|
|NAD (8 mM)||1 x 500µl|
|Recombinant SIRT2||1 x 500µl|
|SIRT Assay Buffer||2 x 1ml|
|Stop Solution||2 x 1ml|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"