Anti-SIRT2 antibody (ab19388)
Key features and details
- Rabbit polyclonal to SIRT2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT2 antibody
See all SIRT2 primary antibodies -
Description
Rabbit polyclonal to SIRT2 -
Host species
Rabbit -
Specificity
SIRT2 antibody (ab19388) detects SIRT2 and shows no cross-reactivity to other SIRT isoforms. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human SIRT2 aa 377-389.
Sequence:DEARTTEREKPQ
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Positive control
- ICC/IF: SAOS2 transfected cell lysates WB: HeLa and MCF7 whole cell lysate; Mouse brain and liver tissue lysates IHC-P: Human heart tissue
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General notes
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
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WB |
1/250. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
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ICC/IF |
1/400.
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Notes |
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IHC-P
1/100. |
WB
1/250. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa). |
ICC/IF
1/400. |
Target
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Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 22933 Human
- Entrez Gene: 64383 Mouse
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q8VDQ8 Mouse
- Unigene: 466693 Human
- Unigene: 272443 Mouse
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Alternative names
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
Images
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All lanes : Anti-SIRT2 antibody (ab19388) at 1/250 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, HRP conjugate at 1/5000 dilution
Predicted band size: 43 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SIRT2 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab19388 diluted in 3% BSA-PBS at a dilution of 1/100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using ab19388.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab19388 has been referenced in 3 publications.
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Lara E et al. Salermide, a Sirtuin inhibitor with a strong cancer-specific proapoptotic effect. Oncogene 28:781-91 (2009). WB ; Human . PubMed: 19060927
- Lovato L et al. Transketolase and CNPase I are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol Cell Proteomics : (2008). WB ; Human . PubMed: 18676363