Anti-SIRT2 antibody (ab67299)
Key features and details
- Rabbit polyclonal to SIRT2
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT2 antibody
See all SIRT2 primary antibodies -
Description
Rabbit polyclonal to SIRT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cynomolgus monkey, Common marmoset, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Whole extracts of mouse and rat brain ICC: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
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ICC |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
ICC
Use at an assay dependent concentration. |
Target
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Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 22933 Human
- Entrez Gene: 64383 Mouse
- Entrez Gene: 361532 Rat
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q8VDQ8 Mouse
- SwissProt: Q5RJQ4 Rat
- Unigene: 466693 Human
see all -
Alternative names
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
Images
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All lanes : Anti-SIRT2 antibody (ab67299) at 2 µg/ml
Lane 1 : Whole extracts of mouse brain
Lane 2 : Whole extracts of rat brain
Lane 3 : Whole extracts of mouse brain with SIRT2 immunizing peptide
Lane 4 : Whole extracts of rat brain with SIRT2 immunizing peptide
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 43 kDachemiluminescent substrate.
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Immunocytochemistry analysis of HeLa cells labeling SIRT2 with ab67299 at 10 μg/mL. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
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ab67299 staining SIRT2 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for 60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation of rSCs with RSV led to an increase of SIRT2 expression in densitometry analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab67299 has been referenced in 19 publications.
- Buscham TJ et al. Progressive axonopathy when oligodendrocytes lack the myelin protein CMTM5. Elife 11:N/A (2022). PubMed: 35274615
- Gargareta VI et al. Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. Elife 11:N/A (2022). PubMed: 35543322
- Jablonska B et al. Sirt2 promotes white matter oligodendrogenesis during development and in models of neonatal hypoxia. Nat Commun 13:4771 (2022). PubMed: 35970992
- Lu W et al. SUMOylation is essential for Sirt2 tumor-suppressor function in neuroblastoma. Neoplasia 23:129-139 (2021). PubMed: 33316537
- Zhou F et al. SIRT2 ablation inhibits glucose-stimulated insulin secretion through decreasing glycolytic flux. Theranostics 11:4825-4838 (2021). PubMed: 33754030