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    sirt2-antibody-ep1668y-ab51023.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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RecombinantRabMAb

Recombinant Anti-SIRT2 antibody [EP1668Y] (ab51023)

  • Datasheet
Reviews (4) Submit a question References (10)

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Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)
  • Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)
  • Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)
  • Flow Cytometry (Intracellular) - Anti-SIRT2 antibody [EP1668Y] (ab51023)
  • Anti-SIRT2 antibody [EP1668Y] (ab51023)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1668Y] to SIRT2
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Alexa Fluor® 647 Anti-SIRT2 antibody [EP1668Y] (ab205831)

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Overview

  • Product name

    Anti-SIRT2 antibody [EP1668Y]
    See all SIRT2 primary antibodies
  • Description

    Rabbit monoclonal [EP1668Y] to SIRT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt (intra): HeLa cells IP: HeLa cell lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1668Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-SIRT2 antibody [EP1668Y] (ab205830)
    • Alexa Fluor® 647 Anti-SIRT2 antibody [EP1668Y] (ab205831)
    • Anti-SIRT2 antibody [EP1668Y] - BSA and Azide free (ab239838)
  • Assay kits

    • SIRT2 Activity Assay Kit (Fluorometric) (ab156066)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human SIRT2 protein (ab138791)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (3)
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
IP (1)
1/50.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
IP
1/50.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • Tissue specificity

    Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • Sequence similarities

    Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • Developmental stage

    Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • Post-translational
    modifications

    Phosphorylated at the G2/M transition of the cell cycle.
  • Cellular localization

    Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Target information above from: UniProt accession Q8IXJ6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 22933 Human
    • Entrez Gene: 361532 Rat
    • Omim: 604480 Human
    • SwissProt: Q8IXJ6 Human
    • SwissProt: Q5RJQ4 Rat
    • Unigene: 466693 Human
    • Unigene: 59887 Rat
    • Alternative names

      • FLJ35621 antibody
      • FLJ37491 antibody
      • NAD dependent deacetylase sirtuin 2 antibody
      • NAD dependent protein deacetylase sirtuin 2 antibody
      • NAD-dependent deacetylase sirtuin-2 antibody
      • NAD-dependent protein deacetylase sirtuin-2 antibody
      • Regulatory protein SIR2 homolog 2 antibody
      • Silencing information regulator 2 like antibody
      • Silent information regulator 2 antibody
      • SIR2 antibody
      • SIR2 like protein 2 antibody
      • Sir2 related protein type 2 antibody
      • SIR2, S. cerevisiae, homolog-loke 2 antibody
      • SIR2-like protein 2 antibody
      • SIR2L antibody
      • SIR2L2 antibody
      • SIRT2 antibody
      • SIRT2_HUMAN antibody
      • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
      • Sirtuin 2 antibody
      • Sirtuin type 2 antibody
      see all

    Images

    • Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)
      Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)
      Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 43 kDa
      Observed band size: 36 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)
      Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)

      Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).

    • Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)
      Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)

      Purified ab51023 at 1/50 dilution (2µg) immunoprecipitating SIRT2 in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab51023 + HeLa whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51023 in HeLa whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 36-43 kDa

    • Flow Cytometry (Intracellular) - Anti-SIRT2 antibody [EP1668Y] (ab51023)
      Flow Cytometry (Intracellular) - Anti-SIRT2 antibody [EP1668Y] (ab51023)

      Intracellular Flow Cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red) (ab51023) or a rabbit IgG (negative) (green). 

       

    • Anti-SIRT2 antibody [EP1668Y] (ab51023)
      Anti-SIRT2 antibody [EP1668Y] (ab51023)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (10)

    Publishing research using ab51023? Please let us know so that we can cite the reference in this datasheet.

    ab51023 has been referenced in 10 publications.

    • Martínez-Gutiérrez A  et al. Shikimic acid protects skin cells from UV-induced senescence through activation of the NAD+-dependent deacetylase SIRT1. Aging (Albany NY) 13:12308-12333 (2021). PubMed: 33901008
    • Zhao Y  et al. SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy. Aging Cell 20:e13391 (2021). PubMed: 34053152
    • Li T  et al. SIRT1/2 orchestrate acquisition of DNA methylation and loss of histone H3 activating marks to prevent premature activation of inflammatory genes in macrophages. Nucleic Acids Res 48:665-681 (2020). PubMed: 31799621
    • Wang C  et al. Acetylation Stabilizes Phosphoglycerate Dehydrogenase by Disrupting the Interaction of E3 Ligase RNF5 to Promote Breast Tumorigenesis. Cell Rep 32:108021 (2020). PubMed: 32783943
    • Yu H  et al. Screening Analysis of Sirtuins Family Expression on Anti-Inflammation of Resveratrol in Endothelial Cells. Med Sci Monit 25:4137-4148 (2019). PubMed: 31158122
    • Nevoral J  et al. Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression. J Anim Sci Biotechnol 10:67 (2019). PubMed: 31413827
    • Du Y  et al. Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression. Mol Med Rep 15:1638-1646 (2017). ICC/IF ; Human . PubMed: 28259910
    • Cha Y  et al. Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Nat Cell Biol 19:445-456 (2017). WB ; Human . PubMed: 28436968
    • van Leeuwen IM  et al. Modulation of p53 C-terminal acetylation by mdm2, p14ARF, and cytoplasmic SirT2. Mol Cancer Ther 12:471-80 (2013). Human . PubMed: 23416275
    • Liu Y  et al. Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56. J Biol Chem 287:41469-80 (2012). WB . PubMed: 22982396

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-SIRT2 antibody [EP1668Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (fibroblast cell)
    Total protein in input
    500 µg
    Specification
    fibroblast cell
    Immuno-precipitation step
    Protein A/G
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 02 2013

    Western blot abreview for Anti-SIRT2 antibody [EP1668Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse fibroblast)
    Loading amount
    50 µg
    Specification
    mouse fibroblast
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 07 2013

    Western blot abreview for Anti-SIRT2 antibody [EP1668Y]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colon cancer cell line and MEF cell line)
    Loading amount
    60 µg
    Specification
    HCT116 colon cancer cell line and MEF cell line
    Treatment
    DMSO control and 2 µM SAHA for 24 hrs
    Gel Running Conditions
    Reduced Denaturing (13%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Christian Marx

    Verified customer

    Submitted Feb 13 2013

    Western blot abreview for Anti-SIRT2 antibody [EP1668Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (fibroblast cell)
    Loading amount
    50 µg
    Specification
    fibroblast cell
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 23 2012

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