Recombinant Anti-SIRT2 antibody [EP1668Y] (ab51023)
![Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62859-anti-sirt2-antibody-ep1668y-western-blot.jpg "Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1668Y] to SIRT2
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SIRT2 antibody [EP1668Y]
See all SIRT2 primary antibodies -
Description
Rabbit monoclonal [EP1668Y] to SIRT2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt (intra): HeLa cells IP: HeLa cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1668Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (3) |
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
|
| IP | (1) |
1/50.
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa). |
|
IP
1/50. |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 22933 Human
- Entrez Gene: 361532 Rat
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q5RJQ4 Rat
- Unigene: 466693 Human
- Unigene: 59887 Rat
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Alternative names
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
Images
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Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-255430-anti-sirt2-antibody-ep1668y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).
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![Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-6-anti-sirt2-antibody-ep1668y-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)Purified ab51023 at 1/50 dilution (2µg) immunoprecipitating SIRT2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab51023 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51023 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36-43 kDa -
![Flow Cytometry (Intracellular) - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62857-anti-sirt2-antibody-ep1668y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SIRT2 antibody [EP1668Y] (ab51023)Intracellular Flow Cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red) (ab51023) or a rabbit IgG (negative) (green).
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![Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-5-benefits-of-recombinant-antibodies.png)
Anti-SIRT2 antibody [EP1668Y] (ab51023)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (10)
ab51023 has been referenced in 10 publications.
- Martínez-Gutiérrez A et al. Shikimic acid protects skin cells from UV-induced senescence through activation of the NAD+-dependent deacetylase SIRT1. Aging (Albany NY) 13:12308-12333 (2021). PubMed: 33901008
- Zhao Y et al. SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy. Aging Cell 20:e13391 (2021). PubMed: 34053152
- Li T et al. SIRT1/2 orchestrate acquisition of DNA methylation and loss of histone H3 activating marks to prevent premature activation of inflammatory genes in macrophages. Nucleic Acids Res 48:665-681 (2020). PubMed: 31799621
- Wang C et al. Acetylation Stabilizes Phosphoglycerate Dehydrogenase by Disrupting the Interaction of E3 Ligase RNF5 to Promote Breast Tumorigenesis. Cell Rep 32:108021 (2020). PubMed: 32783943
- Yu H et al. Screening Analysis of Sirtuins Family Expression on Anti-Inflammation of Resveratrol in Endothelial Cells. Med Sci Monit 25:4137-4148 (2019). PubMed: 31158122
- Nevoral J et al. Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression. J Anim Sci Biotechnol 10:67 (2019). PubMed: 31413827
- Du Y et al. Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression. Mol Med Rep 15:1638-1646 (2017). ICC/IF ; Human . PubMed: 28259910
- Cha Y et al. Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Nat Cell Biol 19:445-456 (2017). WB ; Human . PubMed: 28436968
- van Leeuwen IM et al. Modulation of p53 C-terminal acetylation by mdm2, p14ARF, and cytoplasmic SirT2. Mol Cancer Ther 12:471-80 (2013). Human . PubMed: 23416275
- Liu Y et al. Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56. J Biol Chem 287:41469-80 (2012). WB . PubMed: 22982396
