Recombinant Anti-SIRT2 antibody [EP1668Y] (ab51023)
- Datasheet
- References (5)
- Protocols
Overview
-
Product name
Anti-SIRT2 antibody [EP1668Y]
See all SIRT2 primary antibodies -
Description
Rabbit monoclonal [EP1668Y] to SIRT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
-
Positive control
- WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt: HeLa cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1668Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Assay kits
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab51023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa). | |
| IP | 1/50. | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | 1/100 - 1/250. |
Target
-
Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
-
Database links
- Entrez Gene: 22933 Human
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- Unigene: 466693 Human
-
Alternative names
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
Images
-
![Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62859-anti-sirt2-antibody-ep1668y-western-blot.jpg)
Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-255430-anti-sirt2-antibody-ep1668y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).
-
![Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62857-anti-sirt2-antibody-ep1668y-flow-cytometry.jpg)
Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).
Datasheets and documents
References
This product has been referenced in:
- Yu H et al. Screening Analysis of Sirtuins Family Expression on Anti-Inflammation of Resveratrol in Endothelial Cells. Med Sci Monit 25:4137-4148 (2019). Read more (PubMed: 31158122) »
- Du Y et al. Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression. Mol Med Rep 15:1638-1646 (2017). ICC/IF ; Human . Read more (PubMed: 28259910) »
