Recombinant Anti-SIRT2 antibody [EP1668Y] (ab51023)
Overview
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Product name
Anti-SIRT2 antibody [EP1668Y]
See all SIRT2 primary antibodies -
Description
Rabbit monoclonal [EP1668Y] to SIRT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1668Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab51023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa). | |
| IP | 1/50. | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | 1/100 - 1/250. |
Target
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Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 22933 Human
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- Unigene: 466693 Human
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Alternative names
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
Images
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![Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62859-anti-sirt2-antibody-ep1668y-western-blot.jpg)
Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-255430-anti-sirt2-antibody-ep1668y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).
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![Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62857-anti-sirt2-antibody-ep1668y-flow-cytometry.jpg)
Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab51023 has been referenced in 5 publications.
- Yu H et al. Screening Analysis of Sirtuins Family Expression on Anti-Inflammation of Resveratrol in Endothelial Cells. Med Sci Monit 25:4137-4148 (2019). PubMed: 31158122
- Du Y et al. Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression. Mol Med Rep 15:1638-1646 (2017). ICC/IF ; Human . PubMed: 28259910
- Cha Y et al. Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Nat Cell Biol 19:445-456 (2017). WB ; Human . PubMed: 28436968
- van Leeuwen IM et al. Modulation of p53 C-terminal acetylation by mdm2, p14ARF, and cytoplasmic SirT2. Mol Cancer Ther 12:471-80 (2013). Human . PubMed: 23416275
- Liu Y et al. Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56. J Biol Chem 287:41469-80 (2012). WB . PubMed: 22982396
