Anti-SIRT2 antibody [EP1668Y] (ab51023)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-SIRT2 antibody [EP1668Y]
See all SIRT2 primary antibodies -
DescriptionRabbit monoclonal [EP1668Y] to SIRT2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt: HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1668Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Assay kits
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa). | |
| IP | 1/50. | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/100 - 1/250. |
Target
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FunctionNAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
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Tissue specificityWidely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
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Sequence similaritiesBelongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stagePeaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
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Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localizationCytoplasm > cytoskeleton. Colocalizes with microtubules.
- Information by UniProt
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Database links
- Entrez Gene: 22933 Human
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- Unigene: 466693 Human
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Alternative names
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
Images
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![Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023_1.bmp)
Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-255430-51023ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).
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![Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023_3.jpg)
Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).
Datasheets and documents
References
This product has been referenced in:
- Du Y et al. Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression. Mol Med Rep 15:1638-1646 (2017). ICC/IF ; Human . Read more (PubMed: 28259910) »
- Cha Y et al. Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Nat Cell Biol 19:445-456 (2017). WB ; Human . Read more (PubMed: 28436968) »
