Recombinant
RabMAb

Recombinant Anti-SIRT2 antibody [EP1668Y] - BSA and Azide free (ab239838)

Overview

  • Product name

    Anti-SIRT2 antibody [EP1668Y] - BSA and Azide free
    See all SIRT2 primary antibodies
  • Description

    Rabbit monoclonal [EP1668Y] to SIRT2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab239838 is the carrier-free version of ab51023. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239838 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
    • Tissue specificity

      Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
    • Sequence similarities

      Belongs to the sirtuin family.
      Contains 1 deacetylase sirtuin-type domain.
    • Developmental stage

      Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
    • Post-translational
      modifications

      Phosphorylated at the G2/M transition of the cell cycle.
    • Cellular localization

      Cytoplasm > cytoskeleton. Colocalizes with microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ35621 antibody
      • FLJ37491 antibody
      • NAD dependent deacetylase sirtuin 2 antibody
      • NAD dependent protein deacetylase sirtuin 2 antibody
      • NAD-dependent deacetylase sirtuin-2 antibody
      • NAD-dependent protein deacetylase sirtuin-2 antibody
      • Regulatory protein SIR2 homolog 2 antibody
      • Silencing information regulator 2 like antibody
      • Silent information regulator 2 antibody
      • SIR2 antibody
      • SIR2 like protein 2 antibody
      • Sir2 related protein type 2 antibody
      • SIR2, S. cerevisiae, homolog-loke 2 antibody
      • SIR2-like protein 2 antibody
      • SIR2L antibody
      • SIR2L2 antibody
      • SIRT2 antibody
      • SIRT2_HUMAN antibody
      • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
      • Sirtuin 2 antibody
      • Sirtuin type 2 antibody
      see all

    Images

    • Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51023).

    • Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51023).

    References

    ab239838 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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