Overview

  • Product name

    Anti-SIRT2 antibody [EPR1667]
    See all SIRT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR1667] to SIRT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N terminus of Human SIRT2 (UniProt Q8IXJ6).

  • Positive control

    • SKBR3 and SH-SY5Y cell lysates
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
    • Tissue specificity

      Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
    • Sequence similarities

      Belongs to the sirtuin family.
      Contains 1 deacetylase sirtuin-type domain.
    • Developmental stage

      Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
    • Post-translational
      modifications

      Phosphorylated at the G2/M transition of the cell cycle.
    • Cellular localization

      Cytoplasm > cytoskeleton. Colocalizes with microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ35621 antibody
      • FLJ37491 antibody
      • NAD dependent deacetylase sirtuin 2 antibody
      • NAD dependent protein deacetylase sirtuin 2 antibody
      • NAD-dependent deacetylase sirtuin-2 antibody
      • NAD-dependent protein deacetylase sirtuin-2 antibody
      • Regulatory protein SIR2 homolog 2 antibody
      • Silencing information regulator 2 like antibody
      • Silent information regulator 2 antibody
      • SIR2 antibody
      • SIR2 like protein 2 antibody
      • Sir2 related protein type 2 antibody
      • SIR2, S. cerevisiae, homolog-loke 2 antibody
      • SIR2-like protein 2 antibody
      • SIR2L antibody
      • SIR2L2 antibody
      • SIRT2 antibody
      • SIRT2_HUMAN antibody
      • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
      • Sirtuin 2 antibody
      • Sirtuin type 2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: SIRT2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: SH-SY5Y whole cell lysate (20 µg)


      Lanes 1 - 4: Merged signal (red and green). Green - ab134171 observed at 48 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab134171 was shown to specifically react with SIRT2 in wild-type HAP1 cells. No band was observed when SIRT2 knockout samples were examined. Wild-type and SIRT2 knockout samples were subjected to SDS-PAGE. Ab134171 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

       

    • All lanes : Anti-SIRT2 antibody [EPR1667] (ab134171) at 1/500 dilution

      Lane 1 : SKBR3 cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 43 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Rat Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Sep 05 2013

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