Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1667] to SIRT2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Product nameAnti-SIRT2 antibody [EPR1667]
See all SIRT2 primary antibodies
DescriptionRabbit monoclonal [EPR1667] to SIRT2
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues on the N terminus of Human SIRT2 (UniProt Q8IXJ6).
- SKBR3 and SH-SY5Y cell lysates
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 43 kDa.|
FunctionNAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
Tissue specificityWidely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
Sequence similaritiesBelongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Developmental stagePeaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
modificationsPhosphorylated at the G2/M transition of the cell cycle.
Cellular localizationCytoplasm > cytoskeleton. Colocalizes with microtubules.
- Information by UniProt
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: SIRT2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: SH-SY5Y whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab134171 observed at 48 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab134171 was shown to specifically react with SIRT2 in wild-type HAP1 cells. No band was observed when SIRT2 knockout samples were examined. Wild-type and SIRT2 knockout samples were subjected to SDS-PAGE. Ab134171 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-SIRT2 antibody [EPR1667] (ab134171) at 1/500 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
ab134171 has been referenced in 2 publications.