Overview

  • Product name

    Anti-SIRT2 antibody [EPR20411-105]
    See all SIRT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20411-105] to SIRT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human SIRT2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8IXJ6

  • Positive control

    • WB: Human fetal brain and brain cortex lysates; Mouse brain and cerebral cortex lysates; Rat brain lysate; MCF7 whole cell lysate. IHC-P: Human, mouse and rat cerebrum tissues. IHC-Fr: Mouse striatum tissue. IP: Human fetal brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 36, 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100.

Anitgen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

IP 1/30.

Target

  • Function

    NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • Tissue specificity

    Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • Sequence similarities

    Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • Developmental stage

    Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • Post-translational
    modifications

    Phosphorylated at the G2/M transition of the cell cycle.
  • Cellular localization

    Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD dependent protein deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Images

  • Lane 1 : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/5000 dilution
    Lane 2 : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human brain cortex lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 36,43 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 8 seconds; Lane 2: 1 second.

    SIRT2 exists in two main isoforms, SIRT2.1 (43 kDa) and SIRT2.2 (39 kDa). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:21791548).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse striatum tissue labeling SIRT2 with ab211033 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse stratium is observed [PMID: 17447894]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of human cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • SIRT2 was immunoprecipitated from 0.35 mg of Human fetal brain lysate with ab211033 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211033 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal brain lysate, 10 μg (Input).

    Lane 2: ab211033 IP in Human fetal brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211033 in Human fetal brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse cerebral cortex lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2/3: 5 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:21791548).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of mouse cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of rat cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Hu F  et al. Inhibition of SIRT2 limits tumour angiogenesis via inactivation of the STAT3/VEGFA signalling pathway. Cell Death Dis 10:9 (2018). Read more (PubMed: 30584257) »
See 1 Publication for this product

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