Overview

  • Product name

    SIRT2 Screening Assay Kit
    See all SIRT2 kits
  • Detection method

    Fluorescent
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 16 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 16 3.9%
  • Assay type

    Quantitative
  • Product overview

    Abcam's SIRT2 Screening Assay Kit (ab133082) provides a convenient fluorescence-based method for screening SIRT2 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Gln-Pro-Lys-Lys(epsilon-acetyl)-AMC, is incubated with human recombinant SIRT2 along with its co-substrate NAD+. Deacetylation sensitizes the substrate such that treatment with the Developer in the second step releases a fluorescent product. The Fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests
    96-Well Plate Cover 1 unit
    Half Volume 96-Well Solid Plate (white) 1 unit
    SIRT2 (human recombinant) 2 vials
    SIRT2 Direct Assay Buffer (10X) 1 vial
    SIRT2 Direct Developer 1 vial
    SIRT2 Direct Fluorophore 1 vial
    SIRT2 Direct NAD+ 1 vial
    SIRT2 Direct Nicotinamide 1 vial
    SIRT2 Direct Peptide 2 vials
  • Research areas

  • Function

    NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • Tissue specificity

    Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • Sequence similarities

    Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • Developmental stage

    Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • Post-translational
    modifications

    Phosphorylated at the G2/M transition of the cell cycle.
  • Cellular localization

    Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • Alternative names

    • FLJ35621
    • FLJ37491
    • NAD dependent protein deacetylase sirtuin 2
    • NAD-dependent deacetylase sirtuin-2
    • Regulatory protein SIR2 homolog 2
    • Silent information regulator 2
    • SIR2
    • Sir2 related protein type 2
    • SIR2-like protein 2
    • SIR2L
    • SIR2L2
    • SIRT2
    • SIRT2_HUMAN
    • Sirtuin type 2
    see all

Images

  • Inhibition of SIRT2 by nicotinamide (IC50 = 250 µM)

Protocols

References

ab133082 has not yet been referenced specifically in any publications.

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