Overview

  • Product name
    SIRT3 Activity Assay Kit (Fluorometric)
  • Detection method
    Fluorescent
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Enzyme activity
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s SIRT3 Activity Assay Kit (Fluorometric) (ab156067) detects SIRT3 activity in lysates.

    Primarily, the SIRT3 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT3 inhibitors or activators using crude SIRT3 fraction or purified SIRT3.

    Applications for this kit include:

    1. Screening inhibitors or activators of SIRT3.

    2. Detecting the effects of pharmacological agents on SIRT3.

  • Notes

    Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.

    HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
    Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.

     

Properties

Images

  • Dose dependency curve of recombinant SIRT3 activity
  • Effect of Trichostatin A and NAD on recombinant SIRT3 activity

Protocols

References

This product has been referenced in:
  • Michalak S  et al. Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders. Oxid Med Cell Longev 2017:9703574 (2017). Read more (PubMed: 29081897) »

See 1 Publication for this product

Customer reviews and Q&As

Sirt3 activity in liver of Wistar rart

Good Good 4/5 (Ease of Use)
Abreviews
While Sirt 3 activity was easy to measure with the recombinant enzyme, RFU levels from crude liver lysate of Wistar rats were very low. Finally, after several settings (buffer, fractionation, washing steps, etc...), sirt 3 activity was detected in liver of Wistar rats, under our specific experimental conditions.
Username

Ms. Karine Bardin

Verified customer

Submitted Jul 06 2016



I am happy to confirm that crude cell lysate can be used as samples with this kit but as this often needs a lot of optimisation we would recommend to prepare mitochondria and use a mitochondrial lysate.

I can recommend to use one of...

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1) How specific is the assay? There are two other SIRT proteins in the mitochondria, and both are NAD dependent. It seems as if this assay will measure activity of all of these proteins, no?
I assume you are referring to SIRT5 as the second SIRT pr...

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The recombinant SIRT3 was made/refined from colon bacillus. It is a fragmented protein from the N terminal, plus made to be around 10kDa size (process was not carried out within the cell).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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