Sirt3 activity in liver of Wistar rart

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While Sirt 3 activity was easy to measure with the recombinant enzyme, RFU levels from crude liver lysate of Wistar rats were very low. Finally, after several settings (buffer, fractionation, washing steps, etc...), sirt 3 activity was detected in liver of Wistar rats, under our specific experimental conditions.

Ms. Karine Bardin

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Submitted Jul 06 2016

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