Anti-SIRT3 antibody (ab118334)
Key features and details
- Goat polyclonal to SIRT3
- Suitable for: WB
- Reacts with: Mouse, Caenorhabditis elegans
- Isotype: IgG
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Overview
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Product name
Anti-SIRT3 antibody
See all SIRT3 primary antibodies -
Description
Goat polyclonal to SIRT3 -
Host species
Goat -
Specificity
ab118334 is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Caenorhabditis elegans
Predicted to work with: Rat, Horse, Human, Pig -
Immunogen
Synthetic peptide:
RASGIPASKLVEAH
with a Cysteine residue linker, corresponding to internal sequence amino acids 93-106 of Mouse SIRT3 (NP_071878.2), or amino acids 170-183 (NP_001171275.1). -
Positive control
- Mouse heart lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab118334 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Post-translational
modificationsProcessed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 23410 Human
- Entrez Gene: 64384 Mouse
- Entrez Gene: 293615 Rat
- Omim: 604481 Human
- SwissProt: Q9NTG7 Human
- SwissProt: Q8R104 Mouse
- Unigene: 716456 Human
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Alternative names
- hSIRT 3 antibody
- hSIRT3 antibody
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
see all
Images
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Anti-SIRT3 antibody (ab118334) at 0.3 µg/ml (for 1 hour) + Mouse Heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide.
Datasheets and documents
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Datasheet download
References (10)
ab118334 has been referenced in 10 publications.
- Pezzotta A et al. Low Nephron Number Induced by Maternal Protein Restriction Is Prevented by Nicotinamide Riboside Supplementation Depending on Sirtuin 3 Activation. Cells 11:N/A (2022). PubMed: 36291179
- Bao J et al. circFAM160A2 Promotes Mitochondrial Stabilization and Apoptosis Reduction in Osteoarthritis Chondrocytes by Targeting miR-505-3p and SIRT3. Oxid Med Cell Longev 2021:5712280 (2021). PubMed: 34646424
- Castrejón-Téllez V et al. Effect of a Resveratrol/Quercetin Mixture on the Reversion of Hypertension Induced by a Short-Term Exposure to High Sucrose Levels Near Weaning and a Long-Term Exposure That Leads to Metabolic Syndrome in Rats. Int J Mol Sci 21:N/A (2020). PubMed: 32210194
- Li G et al. a-Linolenic acid but not linolenic acid protects against hypertension: critical role of SIRT3 and autophagic flux. Cell Death Dis 11:83 (2020). PubMed: 32015327
- Bao J et al. Oleanolic Acid Decreases IL-1ß-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-?B Axis in Osteoarthritis. Oxid Med Cell Longev 2020:7517219 (2020). PubMed: 33062145
- Chen N et al. Trilobatin Protects Against Aß25-35-Induced Hippocampal HT22 Cells Apoptosis Through Mediating ROS/p38/Caspase 3-Dependent Pathway. Front Pharmacol 11:584 (2020). PubMed: 32508629
- Castrejón-Téllez V et al. Effect of Sucrose Ingestion at the End of a Critical Window that Increases Hypertension Susceptibility on Peripheral Mechanisms Regulating Blood Pressure in Rats. Role of Sirtuins 1 and 3. Nutrients 11:N/A (2019). PubMed: 30717220
- Chen KH et al. Combined Adipose-Derived Mesenchymal Stem Cells and Low-Energy Extracorporeal Shock Wave Therapy Protect the Brain From Brain Death-Induced Injury in Rat. J Neuropathol Exp Neurol 78:65-77 (2019). PubMed: 30481326
- Hulmi JJ et al. Effects of muscular dystrophy, exercise and blocking activin receptor IIB ligands on the unfolded protein response and oxidative stress. Free Radic Biol Med 99:308-322 (2016). PubMed: 27554968
- Karvinen S et al. Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins. Exp Gerontol 79:46-54 (2016). PubMed: 27038700