Product nameAnti-SIRT3 antibody
See all SIRT3 primary antibodies
DescriptionRabbit polyclonal to SIRT3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse kidney lysate. IHC-P: Human prostate, stomach and esophagus tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class III / Sir2 class
Our Abpromise guarantee covers the use of ab264041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
FunctionNAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain.
modificationsProcessed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- hSIRT 3 antibody
- hSIRT3 antibody
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
Paraffin-embedded human stomach tissue stained for SIRT3 using ab264041 at a 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-SIRT3 antibody (ab264041) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 43 kDa
Blocking buffer: 3% non-fat dry milk in TBST.
Paraffin-embedded human prostate tissue stained for SIRT3 using ab264041 at a 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human esophagus tissue stained for SIRT3 using ab264041 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264041 has not yet been referenced specifically in any publications.