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  1. Link

    sirt3-antibody-ab40963.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-SIRT3 antibody (ab40963)

  • Datasheet
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Western blot - Anti-SIRT3 antibody (ab40963)
  • Immunocytochemistry/ Immunofluorescence - Anti-SIRT3 antibody (ab40963)

Key features and details

  • Rabbit polyclonal to SIRT3
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-SIRT4 antibody (ab10140)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SIRT3 antibody
    See all SIRT3 primary antibodies
  • Description

    Rabbit polyclonal to SIRT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human SIRT3.

    Read Abcam's proprietary immunogen policy (Peptide available as ab45065.)
  • Positive control

    • ab40963 gave a positive signal in Human Liver Tissue Lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class III / Sir2 class
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
Notes
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Post-translational
    modifications

    Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession Q9NTG7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23410 Human
    • Omim: 604481 Human
    • SwissProt: Q9NTG7 Human
    • Unigene: 716456 Human
    • Alternative names

      • hSIRT 3 antibody
      • hSIRT3 antibody
      • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
      • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
      • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
      • Regulatory protein SIR2 homolog 3 antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
      • Sir 2 like 3 antibody
      • SIR 2 like protein 3 antibody
      • SIR 3 antibody
      • SIR2 L3 antibody
      • Sir2 like 3 antibody
      • SIR2 like protein 3 antibody
      • SIR2-like protein 3 antibody
      • SIR2L3 antibody
      • SIR3_HUMAN antibody
      • SIRT 3 antibody
      • SIRT3 antibody
      • Sirtuin 3 antibody
      • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
      • Sirtuin type 3 antibody
      • Sirtuin3 antibody
      see all

    Images

    • Western blot - Anti-SIRT3 antibody (ab40963)
      Western blot - Anti-SIRT3 antibody (ab40963)
      Anti-SIRT3 antibody (ab40963) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?
      Additional bands at: 32 kDa, 36 kDa. We are unsure as to the identity of these extra bands.



      The band observed at 32 kDa is thought to represent the 28 kDa processed form of SIRT3.

    • Immunocytochemistry/ Immunofluorescence - Anti-SIRT3 antibody (ab40963)
      Immunocytochemistry/ Immunofluorescence - Anti-SIRT3 antibody (ab40963)
      ICC/IF image of ab40963 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40963, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab40963? Please let us know so that we can cite the reference in this datasheet.

    ab40963 has not yet been referenced specifically in any publications.

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