Overview

  • Product name

  • Description

    Rabbit polyclonal to SIRT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of mouse SIRT3

Properties

Applications

Our Abpromise guarantee covers the use of ab56214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the sirtuin family. Class I subfamily.
      Contains 1 deacetylase sirtuin-type domain.
    • Post-translational
      modifications

      Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • hSIRT 3 antibody
      • hSIRT3 antibody
      • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
      • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
      • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
      • Regulatory protein SIR2 homolog 3 antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
      • Sir 2 like 3 antibody
      • SIR 2 like protein 3 antibody
      • SIR 3 antibody
      • SIR2 L3 antibody
      • Sir2 like 3 antibody
      • SIR2 like protein 3 antibody
      • SIR2-like protein 3 antibody
      • SIR2L3 antibody
      • SIR3_HUMAN antibody
      • SIRT 3 antibody
      • SIRT3 antibody
      • Sirtuin 3 antibody
      • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
      • Sirtuin type 3 antibody
      • Sirtuin3 antibody
      see all

    Images

    • Anti-SIRT3 antibody (ab56214) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 12 kDa, 35 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      The 48 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SIRT3.

    References

    This product has been referenced in:

    • Giralt A  et al. Peroxisome Proliferator-activated Receptor-{gamma} Coactivator-1{alpha} Controls Transcription of the Sirt3 Gene, an Essential Component of the Thermogenic Brown Adipocyte Phenotype. J Biol Chem 286:16958-66 (2011). WB ; Mouse . Read more (PubMed: 21454513) »
    • Sundaresan NR  et al. SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70. Mol Cell Biol 28:6384-401 (2008). WB ; Mouse . Read more (PubMed: 18710944) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab86671. It will ship Monday for Tuesday delivery.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question
    Answer

    Thank you for contacting Abcam about ab56214. I am sorry to hear that it did not work for you as expected.  I have placed the order for the replacement antibody you requested, ab94345 and you should have it tomorrow morning.  The order # is966637. If there is anything else that I can help you with please let me know.

    Read More

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