• Product name
  • Description
    Rabbit polyclonal to SIRT3
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of mouse SIRT3



Our Abpromise guarantee covers the use of ab56214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the sirtuin family. Class I subfamily.
      Contains 1 deacetylase sirtuin-type domain.
    • Post-translational
      Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • hSIRT 3 antibody
      • hSIRT3 antibody
      • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
      • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
      • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
      • Regulatory protein SIR2 homolog 3 antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
      • Sir 2 like 3 antibody
      • SIR 2 like protein 3 antibody
      • SIR 3 antibody
      • SIR2 L3 antibody
      • Sir2 like 3 antibody
      • SIR2 like protein 3 antibody
      • SIR2-like protein 3 antibody
      • SIR2L3 antibody
      • SIR3_HUMAN antibody
      • SIRT 3 antibody
      • SIRT3 antibody
      • Sirtuin 3 antibody
      • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
      • Sirtuin type 3 antibody
      • Sirtuin3 antibody
      see all


    • Anti-SIRT3 antibody (ab56214) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 12 kDa, 35 kDa. We are unsure as to the identity of these extra bands.

      Exposure time: 20 minutes

      The 48 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SIRT3.


    This product has been referenced in:
    • Giralt A  et al. Peroxisome Proliferator-activated Receptor-{gamma} Coactivator-1{alpha} Controls Transcription of the Sirt3 Gene, an Essential Component of the Thermogenic Brown Adipocyte Phenotype. J Biol Chem 286:16958-66 (2011). WB ; Mouse . Read more (PubMed: 21454513) »
    • Sundaresan NR  et al. SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70. Mol Cell Biol 28:6384-401 (2008). WB ; Mouse . Read more (PubMed: 18710944) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    Thank you for contacting Abcam about ab56214. I am sorry to hear that it did not work for you as expected.  I have placed the order for the replacement antibody you requested, ab94345 and you should have it tomorrow morning.  The orde...

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