Our Abpromise guarantee covers the use of ab75434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).


  • Function

    NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Post-translational

    Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • hSIRT 3 antibody
    • hSIRT3 antibody
    • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
    • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
    • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
    • Regulatory protein SIR2 homolog 3 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
    • Sir 2 like 3 antibody
    • SIR 2 like protein 3 antibody
    • SIR 3 antibody
    • SIR2 L3 antibody
    • Sir2 like 3 antibody
    • SIR2 like protein 3 antibody
    • SIR2-like protein 3 antibody
    • SIR2L3 antibody
    • SIR3_HUMAN antibody
    • SIRT 3 antibody
    • SIRT3 antibody
    • Sirtuin 3 antibody
    • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
    • Sirtuin type 3 antibody
    • Sirtuin3 antibody
    see all


  • Anti-SIRT3 antibody (ab75434) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 16 kDa, 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    The band observed at 42 kDa could potentially be a cleaved form of SIRT3 due to the presence of a transit peptide.


This product has been referenced in:

  • Takakura K  et al. Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions. Heliyon 3:e00462 (2017). WB ; Rat . Read more (PubMed: 29264419) »
  • Li S  et al. p53-Induced growth arrest is regulated by the mitochondrial SirT3 deacetylase. PLoS One 5:e10486 (2010). WB ; Human . Read more (PubMed: 20463968) »
See all 2 Publications for this product

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