Name: Anti-SIRT3 antibody [EPR19755]
Brand: RabMAb
SKU: ab217319
Rating: 4 (4 reviews)

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Western blot - Anti-SIRT3 antibody [EPR19755] (ab217319)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19755] to SIRT3
Suitable for: WB, IHC-P
Reacts with: Human

Carrier Free

Product name

Anti-SIRT3 antibody [EPR19755]
See all SIRT3 primary antibodies
Description

Rabbit monoclonal [EPR19755] to SIRT3
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, HeLa, HepG2 and HT-29 whole cell lysates; Human fetal brain, fetal heart, fetal kidney and fetal liver lysates. IHC-P: Human liver, hepatocellular carcinoma, colon and colon adenocarcinoma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19755
Isotype

IgG
Research areas

Alternative Versions
- Anti-SIRT3 antibody [EPR19755] - BSA and Azide free (ab223531)
Assay kits
- SIRT3 Activity Assay Kit (Fluorometric) (ab156067)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab217319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(3)	1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 43 kDa).
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Tissue specificity

Widely expressed.
Sequence similarities

Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain.
Post-translational
modifications

Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Cellular localization

Mitochondrion matrix.
Target information above from: UniProt accession Q9NTG7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 23410 Human
- Omim: 604481 Human
- SwissProt: Q9NTG7 Human
- Unigene: 716456 Human
Alternative names
- hSIRT 3 antibody
- hSIRT3 antibody
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
- NAD dependent deacetylase sirtuin 3 mitochondrial antibody
- NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
- Regulatory protein SIR2 homolog 3 antibody
- Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
- Sir 2 like 3 antibody
- SIR 2 like protein 3 antibody
- SIR 3 antibody
- SIR2 L3 antibody
- Sir2 like 3 antibody
- SIR2 like protein 3 antibody
- SIR2-like protein 3 antibody
- SIR2L3 antibody
- SIR3_HUMAN antibody
- SIRT 3 antibody
- SIRT3 antibody
- Sirtuin 3 antibody
- Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
- Sirtuin type 3 antibody
- Sirtuin3 antibody
see all

Western blot - Anti-SIRT3 antibody [EPR19755] (ab217319)

All lanes : Anti-SIRT3 antibody [EPR19755] (ab217319) at 1/1000 dilution

Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID:12186850).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] (ab217319)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

According to the literature [PMID: 24774224]: IHC and WB studies both showed a decreased expression of SIRT3 in tumor tissues compared with peritumor tissues. Our IHC-P results also showed decreased expression on about 58% of hepatocellular carcinoma.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] (ab217319)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] (ab217319)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] (ab217319)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human well-differentiated colon adenocarcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Our IHC-P results show that about 75% of well-differentiated colon carcinoma show increased expression.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody [EPR19755] (ab217319)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue labeling SIRT3 with ab217319 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human poor- differentiated colon adenocarcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

According to the literature [PMID: 25105144]: SIRT3 expression level was found to be significantly associated with the lymph node metastasis and tumor stages. Our IHC-P results show that about 25% of poor-differentiated colon carcinoma show decreased expression.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-SIRT3 antibody [EPR19755] (ab217319)

All lanes : Anti-SIRT3 antibody [EPR19755] (ab217319) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-3: 3 minutes; Lane 4: 15 seconds.
Anti-SIRT3 antibody [EPR19755] (ab217319)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab217319? Please let us know so that we can cite the reference in this datasheet.

ab217319 has been referenced in 8 publications.

Wang Y et al. Sirt3 promotes the autophagy of HK-2 human proximal tubular epithelial cells via the inhibition of Notch-1/Hes-1 signaling. Mol Med Rep 24:N/A (2021). PubMed: 34278469
He Q et al. Associations of sirtuins with clinicopathological variables and prognosis in human ovarian cancer. Oncol Lett 19:3278-3288 (2020). PubMed: 32256823
Wang R et al. Sirt3 promotes hepatocellular carcinoma cells sensitivity to regorafenib through the acceleration of mitochondrial dysfunction. Arch Biochem Biophys 689:108415 (2020). PubMed: 32562663
Han WM et al. Acacetin Protects Against High Glucose-Induced Endothelial Cells Injury by Preserving Mitochondrial Function via Activating Sirt1/Sirt3/AMPK Signals. Front Pharmacol 11:607796 (2020). PubMed: 33519472
Hou L et al. ABT737 enhances ovarian cancer cells sensitivity to cisplatin through regulation of mitochondrial fission via Sirt3 activation. Life Sci 232:116561 (2019). PubMed: 31247208
Gao J et al. Lithocarpus polystachyus Rehd. leaves aqueous extract protects against hydrogen peroxide-induced SH-SY5Y cells injury through activation of Sirt3 signaling pathway. Int J Mol Med 42:3485-3494 (2018). PubMed: 30320335
Xu WY et al. Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer. World J Gastroenterol 24:4893-4905 (2018). PubMed: 30487699
Lu AQ et al. Upregulation of miR-137 reverses sorafenib resistance and cancer-initiating cell phenotypes by degrading ANT2 in hepatocellular carcinoma. Oncol Rep 37:2071-2078 (2017). PubMed: 28350139

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (SHSY5Y cells)

Gel Running Conditions

Reduced Denaturing (4-20% tris-glycin gel)

Loading amount

40 µg

Specification

SHSY5Y cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 12 2021

Application

Western blot

Sample

Mouse Tissue lysate - whole (Balb/C mice brain)

Gel Running Conditions

Reduced Denaturing (4-20% tris-glycin gel)

Loading amount

40 µg

Specification

Balb/C mice brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 12 2021

Application

Immunohistochemistry free floating

Sample

Human Tissue sections (Brain (cortex))

Specification

Brain (cortex)

Abcam user community

Verified customer

Submitted Nov 20 2020

Application

Western blot

Sample

Guinea pig Tissue lysate - other (fetal heart)

Gel Running Conditions

Reduced Denaturing (4-15% precast gradient gel)

Loading amount

20 µg

Specification

fetal heart

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 23 2019

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (8)

Customer reviews and Q&As

Western blot abreview for Anti-SIRT3 antibody [EPR19755]

Western blot abreview for Anti-SIRT3 antibody [EPR19755]

Immunohistochemistry free floating abreview for Anti-SIRT3 antibody [EPR19755]

Western blot abreview for Anti-SIRT3 antibody [EPR19755]

Post-translational
modifications