Anti-SIRT5 antibody (ab13697)


  • Product name
  • Description
    Rabbit polyclonal to SIRT5
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 30-46 of Human SIRT5.

  • Positive control
    • Human rat or mouse intestine tissue lysate.



Our Abpromise guarantee covers the use of ab13697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 36 kDa.


  • Function
    NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Domain
    In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • Cellular localization
    Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NAD dependent deacetylase sirtuin 5 antibody
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
    • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
    • Regulatory protein SIR2 homolog 5 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
    • Sir2 like 5 antibody
    • SIR2-like protein 5 antibody
    • SIR2L5 antibody
    • SIR5_HUMAN antibody
    • Sirt5 antibody
    • Sirtuin 5 antibody
    • Sirtuin type 5 antibody
    see all


  • Anti-SIRT5 antibody (ab13697) at 1/1000 dilution + Human intestine lysate

    Western blot analysis of SIRT5 using ab13697 at 1:1000 dilution against 20 µg of human intestine lysate.

  • All lanes : Anti-SIRT5 antibody (ab13697) at 2 µg/ml

    Lane 1 : Human intestine cell lysate
    Lane 2 : Mouse intestine cell lysate
    Lane 3 : Rat intestine cell lysate

    Observed band size: 36 kDa (why is the actual band size different from the predicted?)


ab13697 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab108968 with the order number XXX.


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According to our records, ab13697 was proving difficult to use in WB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand ...

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Thank you for your honest feedback. I agree that the vial of ab13697 you received is not specific for SIRT5. As per our Abpromise guarantee, if you have received this product within the last 6 months, I am happy to offer a free replacement of any prima...

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Western blot
Mouse Tissue lysate - other (Liver mitochondrial extract)
Loading amount
20 µg
Liver mitochondrial extract
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
LiCOR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Michelle Green

Verified customer

Submitted Aug 08 2012

Thank you for contacting Abcam regarding ab13697.

I am sorry that this antibody is not working as specified on the datasheet. I am happy to offer a replacement or credit for this product.

Unfortunately, I am not able to send samples o...

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Thank you for contacting us.

Your credit note ID is xxx.

As per our order system, both ab13697 and ab90845 have already been refunded in xxx with credit note ID xxx.

The current credit note is for ab125881. Read More

Thank you for your call today and for letting us know about the trouble with these antibodies.

Your credit note ID is ***.

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made...

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Thank you for providing more details of your customer's protocol, below are my recommendations to increase the signal (those are very similar to my recommendations for the other antibodies the customer is having problems with): -as discussed before...

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Thank you for contacting us for technical support. The fact the customer is having the correct results with over-expressed protein reassures us that the antibody works and that in Hek293T cell lysate the protein levels are much lower. We have not h...

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