Overview

  • Product name

  • Description

    Rabbit polyclonal to SIRT5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    CLKMARPSSSMADFRKF

    , corresponding to amino acids 30-46 of Human SIRT5

  • Positive control

    • Kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 34 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Domain

    In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • Cellular localization

    Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • NAD dependent deacetylase sirtuin 5 antibody
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
    • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
    • Regulatory protein SIR2 homolog 5 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
    • Sir2 like 5 antibody
    • SIR2-like protein 5 antibody
    • SIR2L5 antibody
    • SIR5_HUMAN antibody
    • Sirt5 antibody
    • Sirtuin 5 antibody
    • Sirtuin type 5 antibody
    see all

Images

  • ab78982 at 5µg/ml staining SIRT5 in kidney tissue.

References

This product has been referenced in:

  • Luo H  et al. Expression of Sirtuins in the Retinal Neurons of Mice, Rats, and Humans. Front Aging Neurosci 9:366 (2017). WB, IHC-P ; Mouse, Rat, Human . Read more (PubMed: 29249955) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s Team who would be happy to organize this. Distributor name:Bita Lifescience  Original order number:xxxabID of original product: 1 X vial ab78982 Original batch number:1062565  abID of replacement product: ab10140 CCEID:xxxThank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

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Answer

Thank you very much for confirming these details. I would be pleased to replace the antibody with an Sirt4 antibody. If the customer is interested in an antibody for IHC-P, I agree that ab10140, ab105039 and ab115517 are suitable. As mentioned on the datasheet all of these require heat-mediated antigen retrieval. This should preferably performed with citrate buffer at pH6, however, might require optimisation according to the experimental setting, especially in the case of staining PFA-perfused mouse brain sections. As for the Sirt5 antibody ab78982, we do not know if these Sirt4 antibodies would work with a protocol for free floating sections and thus the protocol might require optimisation. Also, these antibodies have so far only been tested with human (and rat) samples, however, due to a high sequence homology we expect them to cross-react with murine Sirt4 as well. Therefore, if the customer is happy to try ab10140 (or one of the others) despite these uncertainties, I would be pleased to arrange for a replacement. Otherwise, I could issue a credit note or refund if preferred. Thank you for your cooperation. I look forward to hearing from you with details of how your customer would like to proceed.

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Answer

Thank you very much for providing this further information. The details you have kindly provided will provide us with vital information for our monitoring of product quality. Unfortunately, this antibody has only been tested with a standard protocol for formalin-fixed paraffin-embedded sections and we are unsure under which conditions it would work for perfusion-fixed free-floating samples. For future experiments I would like to suggest trying ab78982 on samples with and without the post-fixation for 4 hours, as this step may damage the antigen even more, leaving the antigen not retrievable. Having said this however, I would also like to ask whether you could confirm the arrival date of the antibody you received. According to our records it was dispatched on September 16th. If the antibody would have been in transit for some two weeks, there is indeed the possibility that it has been damaged. Therefore, I would be pleased to offer a free of charge replacement, a credit note or refund. I hope this information is helpful, thank you for your cooperation. I look forward to hearing from you with details of when you received the antibody and of how you would like to proceed.

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