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Epigenetics and Nuclear Signaling Nuclear Signaling Pathways NFkB pathway
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SIRT6 Activity Assay Kit (Fluorometric) (ab156068)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (3)

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ab156068 - SIRT6 Activity Assay Kit (Fluorometric)
  • ab156068 - SIRT6 Activity Assay Kit (Fluorometric)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Cell culture extracts, Tissue Extracts

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Overview

  • Product name

    SIRT6 Activity Assay Kit (Fluorometric)
    See all SIRT6 kits
  • Detection method

    Fluorescent
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Enzyme activity
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s SIRT6 Activity Assay Kit (Fluorometric) (ab156068) detects deacetylase activity of recombinant SIRT6.

    Primarily, the SIRT6 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.

    Applications for this kit include:

    1. Screening inhibitors or activators of SIRT6.

    2. Detecting the effects of pharmacological agents on SIRT6.

  • Notes

    Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.

    HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
    Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 100 tests
    Developer 1 x 500µl
    Fluoro-Deacetylated Peptide (0.1 mM) 1 x 100µl
    Fluoro-Substrate Peptide (0.1 mM) 1 x 500µl
    NAD (8 mM) 1 x 500µl
    Recombinant SIRT6 1 x 500µl
    SIRT Assay Buffer 2 x 1ml
    Stop Solution 2 x 1ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class III / Sir2 class
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • Histone acetylation and deacetylation
    • Metabolism
    • Types of disease
    • Obesity
  • Function

    NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • Sequence similarities

    Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Cellular localization

    Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Target information above from: UniProt accession Q8N6T7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 2810449N18Rik
    • AI043036
    • Mono ADP ribosyltransferase sirtuin 6
    • NAD-dependent protein deacetylase sirtuin-6
    • Regulatory protein SIR2 homolog
    • Regulatory protein SIR2 homolog 6
    • SIR2 like 6
    • SIR2 like protein 6
    • Sir2 related protein type 6
    • SIR2-like protein 6
    • SIR2L6
    • SIR6_HUMAN
    • SIRT 6
    • SIRT6
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
    • Sirtuin 6
    • Sirtuin type 6
    • Sirtuin6
    see all
  • Database links

    • Entrez Gene: 51548 Human
    • Omim: 606211 Human
    • SwissProt: Q8N6T7 Human
    • Unigene: 423756 Human

    Associated products

      Images

      • ab156068 - SIRT6 Activity Assay Kit (Fluorometric)
        ab156068 - SIRT6 Activity Assay Kit (Fluorometric)
        Dose dependent curve of recombinant SIRT6 activity
      • ab156068 - SIRT6 Activity Assay Kit (Fluorometric)
        ab156068 - SIRT6 Activity Assay Kit (Fluorometric)

        Effect of different compounds on recombinant SIRT6 activity

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab156068? Please let us know so that we can cite the reference in this datasheet.

      ab156068 has been referenced in 3 publications.

      • Peng L  et al. Deacetylase-independent function of SIRT6 couples GATA4 transcription factor and epigenetic activation against cardiomyocyte apoptosis. Nucleic Acids Res 48:4992-5005 (2020). PubMed: 32239217
      • Yao ZQ  et al. A novel small-molecule activator of Sirtuin-1 induces autophagic cell death/mitophagy as a potential therapeutic strategy in glioblastoma. Cell Death Dis 9:767 (2018). PubMed: 29991742
      • Balestrieri ML  et al. Sirtuin 6 expression and inflammatory activity in diabetic atherosclerotic plaques: effects of incretin treatment. Diabetes 64:1395-406 (2015). PubMed: 25325735

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      can this product (SIRT6 activity assay) be used to measure Sirt6 activity in nuclear extracts. And if the assay substrate is not SIRT6 specific would it be possible to treat nuclear fractions with a selective SIRT1 inhibitor in order to effectively measure SIRT6 activity in nuclear lysate.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 14 2014

      Answer



      I believe it is not possible to use ab156068 to detect specifically the activity of only SIRT6 in endogenous nuclear extracts.

      We do not have any experience of measuring endogenous SIRT6 and I could not find such a reference paper using the kit so far.


      Based on our test result in house, the substrate of our SIRT6 kit is very good for SIRT6, but it is also a good substrate at least for SIRT1.

      And other family members, not only SIRT1, could deacetylate this substrate too. Second, in our experience, SIRT6 activity itself is weaker than the activity of other SIRT family members, eg SIRT1 & SIRT3. Therefore we cannot recommend ab156068 for the measurement of SIRT6 activity in nuclear extracts directly even if using SIRT1 inhibitors.

      A possible way around this problem could be an immunoprecipitation of SIRT6 from the nuclear extracts before using the kit.

      We have ab88494 that is tested and guaranteed for IP in human samples:

      https://www.abcam.com/SIRT6-antibody-ab88494.html (or use the following: https://www.abcam.com/SIRT6-antibody-ab88494.html).

      Read More

      Anja Hoffmann

      Abcam Scientific Support

      Answered on Mar 14 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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