SIRT6 Activity Assay Kit (Fluorometric) (ab156068)

Overview

  • Product name
    SIRT6 Activity Assay Kit (Fluorometric)
  • Detection method
    Fluorescent
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Enzyme activity
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s SIRT6 Activity Assay Kit (Fluorometric) (ab156068) detects deacetylase activity of recombinant SIRT6.

    Primarily, the SIRT6 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.

    Applications for this kit include:

    1. Screening inhibitors or activators of SIRT6.

    2. Detecting the effects of pharmacological agents on SIRT6.

  • Notes

    Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.

    HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
    Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.

     

Properties

Images

  • Dose dependent curve of recombinant SIRT6 activity
  • Effect of different compounds on recombinant SIRT6 activity

Protocols

References

ab156068 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



I believe it is not possible to use ab156068 to detect specifically the activity of only SIRT6 in endogenous nuclear extracts.

We do not have any experience of measuring endogenous SIRT6 and I could not find such a reference paper u...

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