Overview

  • Product name

    SIRT6 Activity Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Enzyme activity
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s SIRT6 Activity Assay Kit (Fluorometric) (ab156068) detects deacetylase activity of recombinant SIRT6.

    Primarily, the SIRT6 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.

    Applications for this kit include:

    1. Screening inhibitors or activators of SIRT6.

    2. Detecting the effects of pharmacological agents on SIRT6.

  • Notes

    Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.

    HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
    Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.

     

  • Platform

    Microplate reader

Properties

Images

  • Dose dependent curve of recombinant SIRT6 activity
  • Effect of different compounds on recombinant SIRT6 activity

Protocols

References

This product has been referenced in:

  • Yao ZQ  et al. A novel small-molecule activator of Sirtuin-1 induces autophagic cell death/mitophagy as a potential therapeutic strategy in glioblastoma. Cell Death Dis 9:767 (2018). Read more (PubMed: 29991742) »
  • Balestrieri ML  et al. Sirtuin 6 expression and inflammatory activity in diabetic atherosclerotic plaques: effects of incretin treatment. Diabetes 64:1395-406 (2015). Read more (PubMed: 25325735) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer



I believe it is not possible to use ab156068 to detect specifically the activity of only SIRT6 in endogenous nuclear extracts.

We do not have any experience of measuring endogenous SIRT6 and I could not find such a reference paper using the kit so far.


Based on our test result in house, the substrate of our SIRT6 kit is very good for SIRT6, but it is also a good substrate at least for SIRT1.

And other family members, not only SIRT1, could deacetylate this substrate too. Second, in our experience, SIRT6 activity itself is weaker than the activity of other SIRT family members, eg SIRT1 & SIRT3. Therefore we cannot recommend ab156068 for the measurement of SIRT6 activity in nuclear extracts directly even if using SIRT1 inhibitors.

A possible way around this problem could be an immunoprecipitation of SIRT6 from the nuclear extracts before using the kit.

We have ab88494 that is tested and guaranteed for IP in human samples:

https://www.abcam.com/index.html?datasheet=88494 (or use the following: https://www.abcam.com/index.html?datasheet=88494).

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