SIRT6 Activity Assay Kit (Fluorometric) (ab156068)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Tissue Extracts
Overview
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Product name
SIRT6 Activity Assay Kit (Fluorometric)
See all SIRT6 kits -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Enzyme activity -
Assay time
1h 00m -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s SIRT6 Activity Assay Kit (Fluorometric) (ab156068) detects deacetylase activity of recombinant SIRT6.
Primarily, the SIRT6 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT6.
2. Detecting the effects of pharmacological agents on SIRT6.
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Notes
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others. -
Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests Developer 1 x 500µl Fluoro-Deacetylated Peptide (0.1 mM) 1 x 100µl Fluoro-Substrate Peptide (0.1 mM) 1 x 500µl NAD (8 mM) 1 x 500µl Recombinant SIRT6 1 x 500µl SIRT Assay Buffer 2 x 1ml Stop Solution 2 x 1ml -
Research areas
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Function
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. -
Sequence similarities
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Cellular localization
Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions. - Information by UniProt
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Alternative names
- 2810449N18Rik
- AI043036
- Mono ADP ribosyltransferase sirtuin 6
see all -
Database links
- Entrez Gene: 51548 Human
- Omim: 606211 Human
- SwissProt: Q8N6T7 Human
- Unigene: 423756 Human
Associated products
Images
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ab156068 - SIRT6 Activity Assay Kit (Fluorometric)Dose dependent curve of recombinant SIRT6 activity
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ab156068 - SIRT6 Activity Assay Kit (Fluorometric)Effect of different compounds on recombinant SIRT6 activity
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab156068 has been referenced in 3 publications.
- Peng L et al. Deacetylase-independent function of SIRT6 couples GATA4 transcription factor and epigenetic activation against cardiomyocyte apoptosis. Nucleic Acids Res 48:4992-5005 (2020). PubMed: 32239217
- Yao ZQ et al. A novel small-molecule activator of Sirtuin-1 induces autophagic cell death/mitophagy as a potential therapeutic strategy in glioblastoma. Cell Death Dis 9:767 (2018). PubMed: 29991742
- Balestrieri ML et al. Sirtuin 6 expression and inflammatory activity in diabetic atherosclerotic plaques: effects of incretin treatment. Diabetes 64:1395-406 (2015). PubMed: 25325735